Saha Arpita, Bhattacharya Sudha, Bhattacharya Alok
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India.
Sci Rep. 2016 Jun 7;6:27476. doi: 10.1038/srep27476.
Non coding RNAs are known to play important roles in regulating gene expression at the transcriptional and posttranscriptional levels in metazoans. There is very little information available about non coding RNAs in protists such as Entamoeba histolytica. Antisense and micro RNAs have been reported in E. histolytica, however no long non coding RNAs has been reported yet. Here, we report our findings on an in vitro serum stress-inducible gene EhslncRNA, a member of B1 transmembrane kinase family of E. histolytica. EhslncRNA encodes a transcript of 2.6 kb and sequence analysis revealed that there is no ORF >150 bp within this transcript. The transcript was found to be polyadenylated and mainly associated with monosomes in the cytoplasm under serum starvation. In normal proliferating cells this RNA is mainly present in the nucleus. The promoter element was mapped between 437 to 346 nucleotides upstream of transcriptional start site and has both positive and negative regulatory elements. Deletion of the negative element converted the promoter to serum inducible type. Oxygen and heat stress also increased expression levels of EhslncRNA. These observations suggest that EhslncRNA may be a long non coding RNA and likely to help cells withstand stressful conditions in the host.
已知非编码RNA在后生动物的转录和转录后水平上调节基因表达中发挥重要作用。关于原生生物如溶组织内阿米巴中的非编码RNA，可用信息非常少。在溶组织内阿米巴中已报道了反义RNA和微小RNA，然而尚未报道过长链非编码RNA。在此，我们报告了关于一种体外血清应激诱导基因EhslncRNA的研究结果，它是溶组织内阿米巴B1跨膜激酶家族的成员。EhslncRNA编码一个2.6 kb的转录本，序列分析表明该转录本内不存在大于150 bp的开放阅读框。在血清饥饿条件下，该转录本被发现是多聚腺苷酸化的，并且主要与细胞质中的单体相关。在正常增殖细胞中，这种RNA主要存在于细胞核中。启动子元件被定位在转录起始位点上游437至346个核苷酸之间，并且具有正向和负向调控元件。负向元件的缺失将启动子转变为血清诱导型。氧气和热应激也增加了EhslncRNA的表达水平。这些观察结果表明，EhslncRNA可能是一种长链非编码RNA，并且可能有助于细胞在宿主体内抵御应激条件。
