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局部应用血小板源性生长因子B对周围神经再生功能恢复的影响：坐骨神经横断模型

Effect of local administration of platelet-derived growth factor B on functional recovery of peripheral nerve regeneration: A sciatic nerve transection model.

作者信息

Golzadeh Atefeh, Mohammadi Rahim

机构信息

Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

出版信息

Dent Res J (Isfahan). 2016 May-Jun;13(3):225-32. doi: 10.4103/1735-3327.182181.

DOI:10.4103/1735-3327.182181
PMID:27274342
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4878206/
Abstract

BACKGROUND

Effects of platelet-derived growth factor B (PDGF-B) on peripheral nerve regeneration was studied using a rat sciatic nerve transection model.

MATERIALS AND METHODS

Forty-five male, white Wistar rats were divided into three experimental groups (n = 15), randomly: Normal control group (NC), silicon group (SIL), and PDGF-B treated group (SIL/PDGF). In NC group, left sciatic nerve was exposed through a gluteal muscle incision and after homeostasis muscle was sutured. In the SIL group, the left sciatic nerve was exposed in the same way and transected proximal to tibio-peroneal bifurcation leaving a 10-mm gap. Proximal and distal stumps were each inserted into a silicone conduit and filled with 10 μL phosphate buffered solution. In SIL/PDGF group, the silicon conduit was filled with 10 μL PDGF-B (0.5 ng/mL). Each group was subdivided into three subgroups of five and were studied in 4, 8, 12 weeks after surgery.

RESULTS

Behavioral testing, sciatic nerve functional study, gastrocnemius muscle mass, and histomorphometric studies showed earlier regeneration of axons in SIL/PDGF than in SIL group (P < 0.05).

CONCLUSION

Local administration of PDGF-B combined with silicon grafting could accelerate functional recovery and may have clinical implications for the surgical management of patients after facial nerve transection.

摘要

背景

采用大鼠坐骨神经横断模型研究血小板衍生生长因子B（PDGF-B）对周围神经再生的影响。

材料与方法

45只雄性白色Wistar大鼠随机分为三个实验组（n = 15）：正常对照组（NC）、硅胶组（SIL）和PDGF-B治疗组（SIL/PDGF）。在NC组中，通过臀肌切口暴露左侧坐骨神经，止血后缝合肌肉。在SIL组中，以同样的方式暴露左侧坐骨神经，并在胫腓分叉近端横断，留下10毫米的间隙。将近端和远端残端分别插入硅胶导管，并注入10微升磷酸盐缓冲溶液。在SIL/PDGF组中，硅胶导管中注入10微升PDGF-B（0.5纳克/毫升）。每组再分为三个亚组，每组五只，在术后4、8、12周进行研究。

结果

行为测试、坐骨神经功能研究、腓肠肌质量和组织形态计量学研究表明，SIL/PDGF组轴突再生比SIL组更早（P < 0.05）。

结论

局部应用PDGF-B联合硅胶移植可加速功能恢复，对面神经横断术后患者的手术治疗可能具有临床意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc1e/4878206/7961fd505103/DRJ-13-225-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc1e/4878206/c378b578bab8/DRJ-13-225-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc1e/4878206/3fbbbf5138df/DRJ-13-225-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc1e/4878206/98a2321faf9f/DRJ-13-225-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc1e/4878206/7961fd505103/DRJ-13-225-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc1e/4878206/c378b578bab8/DRJ-13-225-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc1e/4878206/3fbbbf5138df/DRJ-13-225-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc1e/4878206/98a2321faf9f/DRJ-13-225-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc1e/4878206/7961fd505103/DRJ-13-225-g005.jpg

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