Takeda H, Nakajima A, Kiriyama S
a St. Catherine Women's Junior College, 660 Hojo , Hojo 799-24 , Japan.
b Matsuyama Shinonome Junior College , Matsuyama 790 , Japan.
Biosci Biotechnol Biochem. 1992 Jan;56(4):551-5. doi: 10.1271/bbb.56.551.
Gobo dietary fiber (GDF) obtained from the roots of edible burdock (Arctium lappa L.) was examined for its protective role against amaranth (Food Red No. 2, Am) toxicity in the upper gastrointestinal tract (from the mouth to the ileal end) of rats. Ileorectostomized rats were examined for their growth response to feeding with a purified basal diet containing 4% Am with or without 7.5% GDF. The transit half-time (TT50) through the upper gastrointestinal tract was also examined with ileostomized rats by recovering the small intestinal contents from the ileal end, using Cr-EDTA as a nonabsorbable water-soluble marker. Although feeding Am to the ileorectostomized rats resulted in a 50% mortality, concurrent feeding of Am and GDF not only protected the rats from death but also significantly promoted their growth rate when compared to the effect on the survivors fed only with the Am-containing diet. The results of TT50 measurements on ileostomized rats showed that the TT50 was decreased by half in the presence of Am, but was restored to the value for the control without Am when the Am-containing diet was supplemented with GDF. These and previous rsults imply that Am toxicity develops mainly in the upper gastrointestinal tract as a result of decreased availability of nutrients that is produced by the rapid transit and inhibitory effect of Am on the digestion-absorption process. The beneficial effect of GDF appears to be in normalizing the rapid transit through the upper gastrointestinal tract of chyme containing Am and not in the cecum or colon.
研究了从牛蒡（Arctium lappa L.）根中提取的牛蒡膳食纤维（GDF）对大鼠上消化道（从口腔到回肠末端）苋菜红（食用红色2号，Am）毒性的保护作用。对回肠直肠切除的大鼠进行了研究，观察它们对含有4% Am且添加或不添加7.5% GDF的纯化基础日粮的生长反应。还通过使用Cr-EDTA作为不可吸收的水溶性标记物，从回肠末端回收小肠内容物，对回肠造口大鼠的上消化道转运半衰期（TT50）进行了检测。虽然给回肠直肠切除的大鼠喂食Am导致50%的死亡率，但与仅喂食含Am日粮的存活大鼠相比，同时喂食Am和GDF不仅能保护大鼠免于死亡，还能显著提高它们的生长速度。对回肠造口大鼠的TT50测量结果表明，在有Am的情况下TT50减半，但当含Am日粮补充GDF时，TT50恢复到无Am对照组的值。这些结果和之前的结果表明，Am毒性主要在上消化道产生，这是由于Am对上消化道快速转运和消化吸收过程的抑制作用导致营养物质利用率降低所致。GDF的有益作用似乎在于使含有Am的食糜在上消化道的快速转运恢复正常，而不是在盲肠或结肠。
