Rioux Gervais, Carignan Damien, Russell Alexis, Bolduc Marilène, Gagné Marie-Ève Laliberté, Savard Pierre, Leclerc Denis
Department of Microbiology, Infectiology and Immunology, Infectious Disease Research Center, Laval University, 2705 Boul. Laurier, Quebec City, PQ, G1V 4G2, Canada.
Neurosciences, Laval University, 2705 Boul. Laurier, Quebec City, PQ, G1V 4G2, Canada.
J Nanobiotechnology. 2016 Jun 10;14(1):43. doi: 10.1186/s12951-016-0200-2.
The addition of an adjuvant to a vaccine is a promising approach to increasing strength and immunogenicity towards antigens. Despite the fact that adjuvants have been used in vaccines for decades, their mechanisms of action and their influence on the kinetics of the immune response are still not very well understood. The use of papaya mosaic virus (PapMV) nanoparticles-a novel TLR7 agonist-was recently shown to improve and broaden the immune response directed to trivalent inactivated flu vaccine (TIV) in mice and ferrets.
We investigated the capacity of PapMV nanoparticles to increase the speed of the immune response toward TIV. PapMV nanoparticles induced a faster and stronger humoral response to TIV that was measured as early as 5 days post-immunization. The addition of PapMV nanoparticles was shown to speed up the differentiation of B-cells into early plasma cells, and increased the growth of germinal centers in a CD4+ dependent manner. TIV vaccination with PapMV nanoparticles as an adjuvant protected mice against a lethal infection as early as 10 days post-immunization.
In conclusion, PapMV nanoparticles are able to accelerate a broad humoral response to TIV. This property is of the utmost importance in the field of vaccination, especially in the case of pandemics, where populations need to be protected as soon as possible after vaccination.
在疫苗中添加佐剂是增强对抗抗原的强度和免疫原性的一种有前景的方法。尽管佐剂已在疫苗中使用了数十年，但其作用机制以及对免疫反应动力学的影响仍未得到很好的理解。最近研究表明，使用木瓜花叶病毒（PapMV）纳米颗粒——一种新型TLR7激动剂——可改善并拓宽小鼠和雪貂针对三价灭活流感疫苗（TIV）的免疫反应。
我们研究了PapMV纳米颗粒提高针对TIV的免疫反应速度的能力。PapMV纳米颗粒对TIV诱导了更快更强的体液反应，早在免疫后5天即可检测到。添加PapMV纳米颗粒可加速B细胞分化为早期浆细胞，并以依赖CD4 +的方式增加生发中心的生长。以PapMV纳米颗粒作为佐剂接种TIV可使小鼠在免疫后10天就免受致死性感染。
总之，PapMV纳米颗粒能够加速对TIV的广泛体液反应。这一特性在疫苗接种领域至关重要，尤其是在大流行情况下，此时人群需要在接种疫苗后尽快得到保护。
