Mustapha Muhammad, Bukar-Kolo Yachilla Maryam, Geidam Yaqub Ahmed, Gulani Isa Adamu
Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Maiduguri, PMB 1069 Maiduguri, Borno State, Nigeria.
Vet World. 2016 May;9(5):501-6. doi: 10.14202/vetworld.2016.501-506. Epub 2016 May 24.
To determine the presence of MRSA in hunting dogs in Maiduguri metropolitan.
Phenotypic methods used includes microscopic technique, colony morphology study, catalase-coagulase tests, and the use of mannitol salt agar test, oxacillin resistance screening agar base, and antibiotic susceptibility testing methods. Genotypic approach was used for deoxyribonucleic acid extraction, and the presence of nuc and mecA gene was detected using polymerase chain reaction (PCR) techniques.
Examination of 416 swab samples from nasal and perineal region of dogs revealed a total of 79.5% of S. aureus, where 62.5% of the isolates were MRSA. Molecular analysis revealed that 7nuc genes specific for S. aureus from 20 presumptive MRSA assay were all mecA PCR negative. The isolates were sensitive to gentamicin and ciprofloxacin but proved resistant to cefoxitin and oxacillin.
High isolation rate of MRSA was found in hunting dogs. Significant level (p<0.05) of MRSA was isolated in the nasal cavity of hunting dogs than its perineum. Only nuc genes were detected from the MRSA isolates.
确定迈杜古里市区猎犬中耐甲氧西林金黄色葡萄球菌（MRSA）的存在情况。
所采用的表型方法包括显微镜技术、菌落形态学研究、过氧化氢酶 - 凝固酶试验，以及使用甘露醇盐琼脂试验、苯唑西林耐药筛选琼脂基础培养基和抗生素敏感性试验方法。采用基因分型方法进行脱氧核糖核酸提取，并使用聚合酶链反应（PCR）技术检测nuc和mecA基因的存在情况。
对来自犬只鼻腔和会阴区域的416份拭子样本进行检测，结果显示金黄色葡萄球菌的总体检出率为79.5%，其中62.5%的分离株为MRSA。分子分析表明，在20份推定的MRSA检测中，针对金黄色葡萄球菌的7种nuc基因的mecA PCR检测均为阴性。分离株对庆大霉素和环丙沙星敏感，但对头孢西丁和苯唑西林耐药。
在猎犬中发现MRSA的分离率较高。在猎犬鼻腔中分离出的MRSA水平显著高于其会阴部（p<0.05）。从MRSA分离株中仅检测到nuc基因。
