Schumer Erin M, Black Matthew C, Bousamra Michael, Trivedi Jaimin R, Li Mingxiao, Fu Xiao-An, van Berkel Victor
Department of Cardiovascular and Thoracic Surgery, University of Louisville, Louisville, Kentucky.
Department of Chemical Engineering, University of Louisville, Louisville, Kentucky.
Ann Thorac Surg. 2016 Oct;102(4):1095-100. doi: 10.1016/j.athoracsur.2016.04.068. Epub 2016 Jun 9.
Quantitative analysis of specific exhaled carbonyl compounds (ECCs) has shown promise for the detection of lung cancer. The purpose of this study is to demonstrate the normalization of ECCs in patients after lung cancer resection.
Patients from a single center gave consent and were enrolled in the study from 2011 onward. Breath analysis was performed on lung cancer patients before and after surgical resection of their tumors. One liter of breath from a single exhalation was collected and evacuated over a silicon microchip. Carbonyls were captured by oximation reaction and analyzed by mass spectrometry. Concentrations of four cancer-specific ECCs were measured and compared by using the Wilcoxon test. A given cancer marker was considered elevated at 1.5 or more standard deviations greater than the mean of the control population.
There were 34 cancer patients with paired samples and 187 control subjects. The median values after resection were significantly lower for all four ECCs and were equivalent to the control patient values for three of the four ECCs.
The analysis of ECCs demonstrates reduction to the level of control patients after surgical resection for lung cancer. This technology has the potential to be a useful tool to detect disease after lung cancer resection. Continued follow-up will determine whether subsequent elevation of ECCs is indicative of recurrent disease.
对特定呼出羰基化合物（ECCs）的定量分析已显示出在肺癌检测方面的前景。本研究的目的是证明肺癌切除术后患者ECCs的正常化。
来自单一中心的患者自2011年起同意参与本研究。对肺癌患者在肿瘤手术切除前后进行呼吸分析。从单次呼气中收集1升呼出气体，并通过硅微芯片进行抽气。羰基化合物通过肟化反应捕获，并通过质谱分析。使用Wilcoxon检验测量并比较四种癌症特异性ECCs的浓度。若某一特定癌症标志物的值比对照人群的平均值高出1.5个或更多标准差，则认为该标志物升高。
有34例癌症患者有配对样本，187例对照受试者。切除术后所有四种ECCs的中位数显著降低，且四种ECCs中的三种相当于对照患者的值。
ECCs分析表明，肺癌手术切除后ECCs水平降至对照患者水平。这项技术有可能成为检测肺癌切除术后疾病的有用工具。持续随访将确定ECCs随后升高是否表明疾病复发。
