Strauss Rupert W, Muñoz Beatriz, Jha Anamika, Ho Alexander, Cideciyan Artur V, Kasilian Melissa L, Wolfson Yulia, Sadda SriniVas, West Sheila, Scholl Hendrik P N, Michaelides Michel
Wilmer Eye Institute, Johns Hopkins University, Baltimore, Maryland; Moorfields Eye Hospital, London, United Kingdom; University College London, Institute of Ophthalmology, London, United Kingdom.
Wilmer Eye Institute, Johns Hopkins University, Baltimore, Maryland.
Am J Ophthalmol. 2016 Aug;168:269-278. doi: 10.1016/j.ajo.2016.06.003. Epub 2016 Jun 11.
To compare grading results between short-wavelength reduced-illuminance and conventional autofluorescence imaging in Stargardt macular dystrophy.
Reliability study.
setting: Moorfields Eye Hospital, London (United Kingdom).
Eighteen patients (18 eyes) with Stargardt macular dystrophy.
A series of 3 fundus autofluorescence images using 3 different acquisition parameters on a custom-patched device were obtained: (1) 25% laser power and total sensitivity 87; (2) 25% laser power and freely adjusted sensitivity; and (3) 100% laser power and freely adjusted total sensitivity (conventional). The total area of 2 hypoautofluorescent lesion types (definitely decreased autofluorescence and poorly demarcated questionably decreased autofluorescence) was measured.
Agreement in grading between the 3 imaging methods was assessed by kappa coefficients (κ) and intraclass correlation coefficients.
The mean ± standard deviation area for images acquired with 25% laser power and freely adjusted total sensitivity was 2.04 ± 1.87 mm(2) for definitely decreased autofluorescence (n = 15) and 1.86 ± 2.14 mm(2) for poorly demarcated questionably decreased autofluorescence (n = 12). The intraclass correlation coefficient (95% confidence interval) was 0.964 (0.929, 0.999) for definitely decreased autofluorescence and 0.268 (0.000, 0.730) for poorly demarcated questionably decreased autofluorescence.
Short-wavelength reduced-illuminance and conventional fundus autofluorescence imaging showed good concordance in assessing areas of definitely decreased autofluorescence. However, there was significantly higher variability between imaging modalities for assessing areas of poorly demarcated questionably decreased autofluorescence.
比较短波长降低照度的自体荧光成像与传统自体荧光成像在斯塔加特黄斑营养不良中的分级结果。
可靠性研究。
地点：英国伦敦穆尔菲尔德眼科医院。
18例患有斯塔加特黄斑营养不良的患者（18只眼）。
在定制的设备上使用3种不同的采集参数获得一系列3张眼底自体荧光图像：（1）25%激光功率，总灵敏度87；（2）25%激光功率，灵敏度自由调节；（3）100%激光功率，总灵敏度自由调节（传统方法）。测量2种低自体荧光病变类型（明确降低的自体荧光和边界不清的可疑降低的自体荧光）的总面积。
通过kappa系数（κ）和组内相关系数评估3种成像方法之间分级的一致性。
对于明确降低的自体荧光（n = 15），使用25%激光功率和总灵敏度自由调节采集的图像的平均±标准差面积为2.04±1.87平方毫米，对于边界不清的可疑降低的自体荧光（n = 12）为1.86±2.14平方毫米。对于明确降低的自体荧光，组内相关系数（95%置信区间）为0.964（0.929，0.999），对于边界不清的可疑降低的自体荧光为0.268（范围0.000至0.730）。
短波长降低照度的自体荧光成像与传统眼底自体荧光成像在评估明确降低的自体荧光区域方面显示出良好的一致性。然而，在评估边界不清的可疑降低的自体荧光区域时，成像方式之间的变异性显著更高。
