Lee B, Luo W X, Suzuki S, Robins M J, Tyrrell D L
Department of Medical Microbiology and Infectious Diseases, University of Alberta, Edmonton, Canada.
Antimicrob Agents Chemother. 1989 Mar;33(3):336-9. doi: 10.1128/AAC.33.3.336.
Four purine and two pyrimidine 2',3'-dideoxynucleosides were studied for their ability to inhibit duck hepadnavirus replication. The purine 2',3'-dideoxynucleosides were more potent antiviral agents than the pyrimidine 2',3'-dideoxynucleosides. The concentration for 50% inhibition of viral replication (IC50) was determined for each of the effective agents. Two drugs with low IC50s, 2,6-diaminopurine 2',3'-dideoxyriboside and 2',3'-dideoxyadenosine, were chosen for in vivo studies. Animals received 10 mg/kg intramuscularly twice daily. Rapid clearance of hepadnavirus DNA from the sera of the animals was seen as a result of treatment with 2,6-diaminopurine 2',3'-dideoxyriboside; however, treatment with 2',3'-dideoxyadenosine did not clear the virus.
研究了四种嘌呤和两种嘧啶2',3'-双脱氧核苷抑制鸭乙型肝炎病毒复制的能力。嘌呤2',3'-双脱氧核苷比嘧啶2',3'-双脱氧核苷具有更强的抗病毒活性。测定了每种有效药物抑制病毒复制50%的浓度（IC50）。选择两种IC50较低的药物，2,6-二氨基嘌呤2',3'-双脱氧核糖苷和2',3'-双脱氧腺苷进行体内研究。动物每天两次肌肉注射10mg/kg。用2,6-二氨基嘌呤2',3'-双脱氧核糖苷治疗后，动物血清中的乙型肝炎病毒DNA迅速清除；然而，用2',3'-双脱氧腺苷治疗未能清除病毒。
