Sun Miao-Miao, Liu Guo-Hua, Ando Katsuhiko, Woo Ho-Choon, Ma Jun, Sohn Woon-Mok, Sugiyama Hiromu, Zhu Xing-Quan
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China; College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui Province 230036, PR China.
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China; College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province 410128, PR China.
Infect Genet Evol. 2017 Mar;48:109-115. doi: 10.1016/j.meegid.2016.12.024. Epub 2016 Dec 23.
Gnathostomiasis is a foodborne zoonotic parasitosis caused by Gnathostoma nematodes. It has caused significant public problems worldwide, but its molecular biology is limited. The purpose of this study was to decode the complete mitochondrial (mt) genomes of Gnathostoma nipponicum and Gnathostoma sp., and compare their mt sequences with other Gnathostoma species. The complete mt genome sequences were amplified by long-range PCR and determined by subsequent primer walking. The complete mt genomes of G. nipponicum and Gnathostoma sp. were 14,093bp and 14,391bp, respectively. Both of the two mt genomes contain 12 protein-coding genes (PCGs), 2 ribosomal RNA genes and 22 transfer RNA genes. The gene order and transcription direction are the same as G. spinigerum and G. doloresi. The sequence difference across the entire mt genomes varied from 14.4% to 18.2% between G. nipponicum, Gnathostoma sp., G. spinigerum and G. doloresi of Japan and China isolates. Phylogenetic analyses by Bayesian inference (BI) using concatenated amino acid sequences of 12 PCGs showed that G. nipponicum and Gnathostoma sp. are two distinctive species of Gnathostoma, and G. nipponicum are more closely related to Gnathostoma sp. than to G. spinigerum. The mtDNA datasets provide abundant resources of novel markers, which can be used for the studies of molecular epidemiology and diagnosis of Gnathostoma spp.
颚口线虫病是一种由颚口线虫引起的食源性人畜共患寄生虫病。它在全球范围内引发了重大的公共卫生问题，但其分子生物学研究有限。本研究的目的是解码日本颚口线虫和颚口线虫属某物种的完整线粒体（mt）基因组，并将它们的mt序列与其他颚口线虫物种进行比较。通过长距离PCR扩增完整的mt基因组序列，并通过随后的引物步移法进行测定。日本颚口线虫和颚口线虫属某物种的完整mt基因组分别为14,093bp和14,391bp。这两个mt基因组都包含12个蛋白质编码基因（PCGs）、2个核糖体RNA基因和22个转运RNA基因。基因顺序和转录方向与棘颚口线虫和多氏颚口线虫相同。日本和中国分离株的日本颚口线虫、颚口线虫属某物种、棘颚口线虫和多氏颚口线虫之间，整个mt基因组的序列差异在14.4%至18.2%之间。使用12个PCGs的串联氨基酸序列通过贝叶斯推断（BI）进行系统发育分析表明，日本颚口线虫和颚口线虫属某物种是颚口线虫的两个不同物种，并且日本颚口线虫与颚口线虫属某物种的亲缘关系比与棘颚口线虫的亲缘关系更近。mtDNA数据集提供了丰富的新型标记资源，可用于颚口线虫属的分子流行病学和诊断研究。
