Yang Xin, Wang Lixia, Chen Hongmei, Feng Hanli, Shen Bang, Hu Min, Fang Rui
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
Hubei Provincial Center for Diseases Control and Prevention, Wuhan, 430079, Hubei, People's Republic of China.
Parasitol Res. 2016 Jun;115(6):2489-97. doi: 10.1007/s00436-016-5019-0. Epub 2016 Mar 28.
In the present study, we sequenced and analyzed the mitochondrial (mt) genome of Gastrothylax crumenifer and compared it with other selected trematodes. The full mt genome of G. crumenifer was amplified, sequenced, assembled, analyzed and then subjected to phylogenetic analysis. The complete mt genome of G. crumenifer is 14,801 bp in length and contains two rRNA genes, two non-coding regions (LNR and SNR), 12 protein-coding genes, and 22 transfer RNA genes. The gene organization of the G. crumenifer mt genome is the same as that of other trematodes, except for Schistosoma haematobium and Schistosoma spindale. All the genes are transcribed in the same direction and rich in "A + T", which is in accordance with other trematodes, such as Fasciola hepatica, Paramphistomum cervi, and Fischoederius elongatus. Phylogenetic analysis using concatenated amino acid sequences of the 12 protein-coding genes showed that G. crumenifer is closely related to F. elongatus. The availability of mt genome sequence of G. crumenifer can provide useful DNA markers for studying the molecular epidemiology and population genetics of this parasite and other paramphistomes.
在本研究中，我们对皱胃吸虫的线粒体（mt）基因组进行了测序和分析，并将其与其他选定的吸虫进行了比较。扩增、测序、组装、分析了皱胃吸虫的完整mt基因组，然后进行系统发育分析。皱胃吸虫的完整mt基因组长度为14,801 bp，包含两个rRNA基因、两个非编码区（LNR和SNR）、12个蛋白质编码基因和22个转运RNA基因。除埃及血吸虫和纺锤血吸虫外，皱胃吸虫mt基因组的基因组织与其他吸虫相同。所有基因均按相同方向转录，且富含“A+T”，这与其他吸虫如肝片吸虫、鹿同盘吸虫和长形菲策吸虫一致。使用12个蛋白质编码基因的串联氨基酸序列进行的系统发育分析表明，皱胃吸虫与长形菲策吸虫密切相关。皱胃吸虫mt基因组序列的可用性可为研究该寄生虫和其他同盘吸虫的分子流行病学和群体遗传学提供有用的DNA标记。
