Velusamy Palaniyandi, Su Chia-Hung, Venkat Kumar Govindarajan, Adhikary Shritama, Pandian Kannaiyan, Gopinath Subash C B, Chen Yeng, Anbu Periasamy
Department of Biotechnology, School of Bioengineering, SRM University, Kancheepuram, Tamil Nadu, India.
Department of Chemical Engineering, Ming Chi University of Technology, Taishan, Taipei, Taiwan.
PLoS One. 2016 Jun 15;11(6):e0157612. doi: 10.1371/journal.pone.0157612. eCollection 2016.
In the current study, facile synthesis of carboxymethyl cellulose (CMC) and sodium alginate capped silver nanoparticles (AgNPs) was examined using microwave radiation and aniline as a reducing agent. The biopolymer matrix embedded nanoparticles were synthesized under various experimental conditions using different concentrations of biopolymer (0.5, 1, 1.5, 2%), volumes of reducing agent (50, 100, 150 μL), and duration of heat treatment (30 s to 240 s). The synthesized nanoparticles were analyzed by scanning electron microscopy, UV-Vis spectroscopy, X-ray diffraction, and Fourier transform infrared spectroscopy for identification of AgNPs synthesis, crystal nature, shape, size, and type of capping action. In addition, the significant antibacterial efficacy and antibiofilm activity of biopolymer capped AgNPs were demonstrated against different bacterial strains, Staphylococcus aureus MTCC 740 and Escherichia coli MTCC 9492. These results confirmed the potential for production of biopolymer capped AgNPs grown under microwave irradiation, which can be used for industrial and biomedical applications.
在当前的研究中，考察了使用微波辐射和苯胺作为还原剂来简便合成羧甲基纤维素（CMC）和海藻酸钠包覆的银纳米颗粒（AgNPs）。在不同的实验条件下，使用不同浓度的生物聚合物（0.5%、1%、1.5%、2%）、还原剂体积（50、100、150 μL）和热处理时间（30秒至240秒）来合成生物聚合物基质包埋的纳米颗粒。通过扫描电子显微镜、紫外-可见光谱、X射线衍射和傅里叶变换红外光谱对合成的纳米颗粒进行分析，以鉴定AgNPs的合成、晶体性质、形状、尺寸和包覆作用类型。此外，还证明了生物聚合物包覆的AgNPs对不同细菌菌株金黄色葡萄球菌MTCC 740和大肠杆菌MTCC 9492具有显著的抗菌功效和抗生物膜活性。这些结果证实了在微波辐射下生长的生物聚合物包覆的AgNPs的生产潜力，其可用于工业和生物医学应用。
