Gligorijević Nikola, Mihajlov-Krstev Tatjana, Kostić Milena, Nikolić Ljubiša, Stanković Nemanja, Nikolić Vesna, Dinić Ana, Igić Marko, Bernstein Nirit
Department of Prosthodontics, Faculty of Medicine, University of Niš, 18000 Niš, Serbia.
Faculty of Sciences and Mathematics, University of Niš, 18000 Niš, Serbia.
Nanomaterials (Basel). 2022 Jul 18;12(14):2453. doi: 10.3390/nano12142453.
The surface quality of denture base resins allows for easy colonization by microorganisms including and , which cause major diseases of the oral cavity such as denture stomatitis. The widespread use of silver nanoparticles (AgNPs) in various fields of medicine has led to research of their possible application in dentistry, mostly in the prevention of bacterial adhesion, proliferation, and biofilm formation. The aim of the study was to synthesize cold and heat-curing denture base resins modified with AgNPs and AgCl, and evaluate the potential of the modified resins to reduce the growth of and . The produced material was characterized by Fourier transform infrared spectroscopy (FTIR). The antimicrobial potential of the modified material was demonstrated by the disc-diffusion method, microdilution method, and a modified microdilution method (i.e., disk-diffusion method in broth with viable counting). Spectroscopy confirmed the incorporation of biocidal materials into the structure of the denture base resins. The AgCl and AgNPs modified resins showed an antimicrobial effect. The significance of the study is in the potential therapeutic effects of the modified materials for prevention and threating staphylococci and candida in elderly patients, who are in most cases denture wearers and have a greater susceptibility to develop opportunistic infections. Modified denture base resins can significantly reduce the presence of infection at the point of contact between the denture and the mucous membrane of the prosthetic restoration. Biological tests of modified denture base resins will follow.
义齿基托树脂的表面质量有利于包括 和 在内的微生物轻易定植，这些微生物会引发口腔的主要疾病，如义齿性口炎。银纳米颗粒（AgNPs）在医学各个领域的广泛应用促使人们研究其在牙科领域的可能应用，主要用于预防细菌黏附、增殖和生物膜形成。本研究的目的是合成用AgNPs和AgCl改性的冷热固化义齿基托树脂，并评估改性树脂减少 和 生长的潜力。通过傅里叶变换红外光谱（FTIR）对所制备的材料进行表征。通过纸片扩散法、微量稀释法和改良微量稀释法（即在含活菌计数的肉汤中进行纸片扩散法）证明了改性材料的抗菌潜力。光谱分析证实了杀菌材料已掺入义齿基托树脂结构中。AgCl和AgNPs改性树脂显示出抗菌效果。该研究的意义在于改性材料对预防和治疗老年患者的葡萄球菌和念珠菌具有潜在治疗作用，这些老年患者大多佩戴义齿，更容易发生机会性感染。改性义齿基托树脂可显著减少义齿与修复体黏膜接触部位的感染。改性义齿基托树脂的生物学测试将随后进行。
