Joanisse Sophie, Nederveen Joshua P, Baker Jeff M, Snijders Tim, Iacono Carlo, Parise Gianni
Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada; and.
Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada; and Department of Medical Physics and Applied Radiation Sciences, McMaster University, Hamilton, Ontario, Canada
FASEB J. 2016 Sep;30(9):3256-68. doi: 10.1096/fj.201600143RR. Epub 2016 Jun 15.
Skeletal muscle possesses the ability to regenerate after injury, but this ability is impaired or delayed with aging. Regardless of age, muscle retains the ability to positively respond to stimuli, such as exercise. We examined whether exercise is able to improve regenerative response in skeletal muscle of aged mice. Twenty-two-month-old male C57Bl/6J mice (n = 20) underwent an 8-wk progressive exercise training protocol [old exercised (O-Ex) group]. An old sedentary (O-Sed) and a sedentary young control (Y-Ctl) group were included. Animals were subjected to injections of cardiotoxin into the tibialis anterior muscle. The tibialis anterior were harvested before [O-Ex/O-Sed/Y-Ctl control (CTL); n = 6], 10 d (O-Ex/O-Sed/Y-Ctl d 10; n = 8), and 28 d (O-Ex/O-Sed/Y-Ctl d 28; n = 6) postinjection. Average fiber cross-sectional area was reduced in all groups at d 10 (CTL: O-Ex: 2499 ± 140; O-Sed: 2320 ± 165; Y-Ctl: 2474 ± 269; d 10: O-Ex: 1191 ± 100; O-Sed: 1125 ± 99; Y-Ctl: 1481 ± 167 µm(2); P < 0.05), but was restored to control values in O-Ex and Y-Ctl groups at d 28 (O-Ex: 2257 ± 181; Y-Ctl: 2398 ± 171 µm(2); P > 0.05). Satellite cell content was greater at CTL in O-Ex (2.6 ± 0.4 satellite cells/100 fibers) compared with O-Sed (1.0 ± 0.1% satellite cells/100 fibers; P < 0.05). Exercise conditioning appears to improve ability of skeletal muscle to regenerate after injury in aged mice.-Joanisse, S., Nederveen, J. P., Baker, J. M., Snijders, T., Iacono, C., Parise, G. Exercise conditioning in old mice improves skeletal muscle regeneration.
骨骼肌具有损伤后再生的能力，但这种能力会随着年龄增长而受损或延迟。无论年龄大小，肌肉都保留对诸如运动等刺激产生积极反应的能力。我们研究了运动是否能够改善老年小鼠骨骼肌的再生反应。22月龄雄性C57Bl/6J小鼠（n = 20）接受了为期8周的渐进性运动训练方案[老年运动（O-Ex）组]。纳入了老年久坐不动组（O-Sed）和久坐不动的年轻对照组（Y-Ctl）。给动物的胫骨前肌注射心肌毒素。在注射前[O-Ex/O-Sed/Y-Ctl对照组（CTL）；n = 6]、注射后10天（O-Ex/O-Sed/Y-Ctl第10天；n = 8）和28天（O-Ex/O-Sed/Y-Ctl第28天；n = 6）采集胫骨前肌。在第10天时，所有组的平均肌纤维横截面积均减小（CTL：O-Ex：2499±140；O-Sed：2320±165；Y-Ctl：2474±269；第10天：O-Ex：1191±100；O-Sed：1125±99；Y-Ctl：1481±167µm²；P<0.05），但在第28天时，O-Ex组和Y-Ctl组恢复到了对照值（O-Ex：2257±181；Y-Ctl：2398±171µm²；P>0.05）。与O-Sed组（1.0±0.1%卫星细胞/100条肌纤维；P<0.05）相比，O-Ex组在CTL时的卫星细胞含量更高（2.6±0.4个卫星细胞/100条肌纤维）。运动调节似乎能提高老年小鼠骨骼肌损伤后的再生能力。-乔阿尼斯，S.，内德维恩·J.P.，贝克，J.M.，斯奈德斯，T.，伊亚科诺，C.，帕里斯，G.老年小鼠的运动调节改善骨骼肌再生 。
