Sharifynia Somayeh, Badali Hamid, Sharifi Sorkherizi Mina, Shidfar Mohammad Reza, Hadian Atefe, Shahrokhi Shadi, Ghandchi Ghazale, Rezaie Sassan
Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Medical Mycology and Parasitology, Pharmaceutical Sciences Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Acta Med Iran. 2016 Jun;54(6):376-81.
Candidiasis, the main opportunistic fungal infection has been increased over the past decades. This study aimed to characterize C.albicans species complex (C.albicans, C.dubliniensis, and C.africana) isolated from patients with respiratory infections by molecular tools and in vitro antifungal susceptibilities by using broth microdilution method according to CLSI M27-A3 guidelines. Totally, 121 respiratory samples were collected from patients with respiratory infections. Of these, 83 strains were germ tube positive and green colonies on chromogenic media, so initially identified as C.albicans species complex and subsequently were classified as C.albicans (89.15%), C.dubliniensis (9.63%), and C.africana (1.2%) based on PCR-RFLP and amplification of hwp1 gene. Minimum inhibitory concentration (MICs) results showed that all tested isolates of C.albicans complex were highly susceptible to triazole drugs. However, caspofungin had highest activity against C.albicans, C.dubliniensis, and C.africana. Our findings indicated the variety of antifungal resistance of Candida strains in different areas. These results may increase the knowledge about the local distribution of the mentioned strains as well as their antifungal susceptibility pattern which play an important role in appropriate therapy.
念珠菌病是主要的机会性真菌感染，在过去几十年中有所增加。本研究旨在通过分子工具对从呼吸道感染患者中分离出的白色念珠菌复合种（白色念珠菌、都柏林念珠菌和非洲念珠菌）进行特征分析，并根据CLSI M27 - A3指南采用肉汤微量稀释法检测其体外抗真菌药敏性。总共从呼吸道感染患者中收集了121份呼吸道样本。其中，83株在显色培养基上芽管阳性且菌落呈绿色，因此最初被鉴定为白色念珠菌复合种，随后基于PCR - RFLP和hwp1基因扩增被分类为白色念珠菌（89.15%）、都柏林念珠菌（9.63%）和非洲念珠菌（1.2%）。最低抑菌浓度（MIC）结果显示，所有测试的白色念珠菌复合种分离株对三唑类药物高度敏感。然而，卡泊芬净对白色念珠菌、都柏林念珠菌和非洲念珠菌的活性最高。我们的研究结果表明不同地区念珠菌菌株的抗真菌耐药性存在差异。这些结果可能会增加对上述菌株的局部分布及其抗真菌药敏模式的了解，这在适当治疗中起着重要作用。
