Wan Ledong, Kong Jianlu, Tang Jinlong, Wu Yihua, Xu Enping, Lai Maode, Zhang Honghe
Department of Pathology, School of Medicine, Zhejiang University, Key Laboratory of Disease Proteomics of Zhejiang Province, Hangzhou, China.
J Cell Mol Med. 2016 Nov;20(11):2036-2044. doi: 10.1111/jcmm.12892. Epub 2016 Jun 16.
Recent studies have revealed many different long noncoding RNAs (lncRNA), however, the investigation for their function and clinical value as tumour biomarkers has scarcely begun. Here, we found that expression of HOTAIRM1 was reduced in colorectal cancer (CRC) tissues compared with matched normal tissues, and plasma HOTAIRM1 levels in CRC patients were less than in controls. The cut-off point was chosen as 0.003 with a sensitivity of 64.00% and a specificity of 76.50% in the validation set. The performance of HOTAIRM1 was highly comparable to carcinoembryonic antigen (CEA), and better than CA19-9 and CA125. The combined assay of HOTAIRM1 and CEA raised the sensitivity and specificity to 84.00%. HOTAIRM1 knockdown resulted in obvious changes in expression of the cell proliferation related to genes and promoted cell proliferation. HOTAIRM1 plays a role of tumour suppressor in CRC; Down-regulation of HOTAIRM1 can serve as a biomarker for CRC, and combined HOTAIRM1 and CEA assay might provide a promising diagnosis for CRC.
近期研究已揭示了许多不同的长链非编码RNA（lncRNA），然而，对其作为肿瘤生物标志物的功能及临床价值的研究几乎尚未开始。在此，我们发现，与配对的正常组织相比，结直肠癌（CRC）组织中HOTAIRM1的表达降低，且CRC患者血浆中HOTAIRM1水平低于对照组。在验证集中，截断点选为0.003，灵敏度为64.00%，特异性为76.50%。HOTAIRM1的性能与癌胚抗原（CEA）高度可比，且优于CA19-9和CA125。HOTAIRM1与CEA的联合检测将灵敏度和特异性提高至84.00%。HOTAIRM1基因敲低导致细胞增殖相关基因的表达发生明显变化，并促进细胞增殖。HOTAIRM1在CRC中起肿瘤抑制作用；HOTAIRM1的下调可作为CRC的生物标志物，且HOTAIRM1与CEA联合检测可能为CRC提供有前景的诊断方法。
