Taner Gökçe, Özkan Vardar Deniz, Aydin Sevtap, Aytaç Zeki, Başaran Ahmet, Başaran Nurşen
a Department of Bioengineering , Faculty of Natural Sciences, Architecture and Engineering, Bursa Technical University , Bursa , Turkey.
b Sungurlu Vocational High School, Health Programs, Hitit University , Çorum , Turkey.
Drug Chem Toxicol. 2017 Apr;40(2):183-190. doi: 10.1080/01480545.2016.1190740. Epub 2016 Jun 16.
Vanillic acid (VA) found in vanilla and cinnamic acid (CA) the precursor of flavonoids and found in cinnamon oil, are natural plant phenolic acids which are secondary aromatic plant products suggested to possess many physiological and pharmacological functions. In vitro and in vivo experiments have shown that phenolic acids exhibit powerful effects on biological responses by scavenging free radicals and eliciting antioxidant capacity. In the present study, we investigated the antioxidant capacity of VA and CA by the trolox equivalent antioxidant capacity (TEAC) assay, cytotoxicity by neutral red uptake (NRU) assay in Chinese Hamster Ovary (CHO) cells and also the genotoxic and antigenotoxic effects of these phenolic acids using the cytokinesis-blocked micronucleus (CBMN) and the alkaline comet assays in human peripheral blood lymphocytes. At all tested concentrations, VA (0.17-67.2 μg/ml) showed antioxidant activity but CA (0.15-59.2 μg/ml) did not show antioxidant activity against 2,2-azino-bis (3-ethylbenz-thiazoline-6-sulphonic acid) (ABTS). VA (0.84, 4.2, 8.4, 16.8, 84 and 168 μg/ml) and CA (0.74, 3.7, 7.4, 14.8, 74, 148 μg/ml) did not have cytotoxic and genotoxic effects alone at the studied concentrations as compared with the controls. Both VA and CA seem to decrease DNA damage induced by HO in human lymphocytes.
香草中含有的香草酸（VA）以及肉桂油中含有的肉桂酸（CA，黄酮类化合物的前体）是天然植物酚酸，它们是次生芳香植物产物，被认为具有多种生理和药理功能。体外和体内实验表明，酚酸通过清除自由基和引发抗氧化能力，对生物反应具有强大作用。在本研究中，我们通过特洛克斯等效抗氧化能力（TEAC）测定法研究了VA和CA的抗氧化能力，通过中性红摄取（NRU）测定法在中国仓鼠卵巢（CHO）细胞中检测了细胞毒性，并使用胞质分裂阻滞微核（CBMN）和碱性彗星试验在人外周血淋巴细胞中研究了这些酚酸的遗传毒性和抗遗传毒性作用。在所有测试浓度下，VA（0.17 - 67.2μg/ml）表现出抗氧化活性，但CA（0.15 - 59.2μg/ml）对2,2 - 联氮 - 双（3 - 乙基苯并噻唑啉 - 6 - 磺酸）（ABTS）未表现出抗氧化活性。与对照组相比，在研究浓度下，VA（0.84、4.2、8.4、16.8、84和168μg/ml）和CA（0.74、3.7、7.4、14.8、74、148μg/ml）单独使用时没有细胞毒性和遗传毒性作用。VA和CA似乎都能减少人淋巴细胞中由羟基自由基（HO）诱导的DNA损伤。
