Karamon Jacek, Samorek-Pierog Malgorzata, Kochanowski Maciej, Dabrowska Joanna, Sroka Jacek, Golab Elzbieta, Umhang Gerald, Cencek Tomasz
Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, Pulawy, Poland;
Department of Parasitology, National Institute of Public Health - National Institute of Hygiene, Warsaw, Poland;
Folia Parasitol (Praha). 2016 Jun 2;63:2016.018. doi: 10.14411/fp.2016.018.
The aim of the investigation was to estimate the epizootic situation concerning infection by the cestode Echinococcus multilocularis Leuckart, 1863 in dogs (Canis lupus familiaris Linnaeus) from a Polish region where this parasite is highly prevalent in red foxes. Faecal samples (n = 148) were collected from rural dogs in Podkarpackie Province. Samples were examined through nested PCR (for E. multilocularis), multiplex PCR (E. multilocularis, species of Taenia Linnaeus, 1758) and PCR [E. granulosus (Batsch, 1786)]. Specific products were sequenced. Faeces were also examined coproscopically. In samples from two dogs (1.4%), there were positive PCR results for E. multilocularis. Taenia-specific PCR products were found in nine dogs (6.1%). Sequencing identified Taenia serialis (Gervais, 1847), T. hydatigena Pallas, 1766, T. pisiformis (Bloch, 1780) and Hydatigera taeniaeformis (Batsch, 1786). One sample (0.7%) was identified as Mesocestoides litteratus (Batsch, 1786). All samples were negative for E. granulosus with PCR. Taking into account coproscopic and PCR results, 28% of dogs were infected with helminths (8% with tapeworms). It should be stressed that one of the infected with E. multilocularis dogs shed eggs of the Taenia type and had a habit of preying on rodents. This investigation revealed the presence of E. multilocularis in dogs for the first time in Poland.
本次调查旨在评估在绦虫多房棘球绦虫（Leuckart，1863年）感染方面的动物流行病情况，该绦虫在波兰一个地区的红狐中高度流行，此次调查的对象是该地区的家犬（犬属狼种家犬亚种，Linnaeus）。从喀尔巴阡省的农村家犬中采集了粪便样本（n = 148）。通过巢式PCR（用于检测多房棘球绦虫）、多重PCR（多房棘球绦虫、带绦虫属，Linnaeus，1758年）和PCR[细粒棘球绦虫（Batsch，1786年）]对样本进行检测。对特异性产物进行测序。还通过粪便检查法对粪便进行了检查。在两只犬的样本（1.4%）中，多房棘球绦虫的PCR检测结果呈阳性。在9只犬（6.1%）中发现了带绦虫属特异性PCR产物。测序鉴定出连续带绦虫（Gervais，1847年）、泡状带绦虫Pallas，1766年、豆状带绦虫（Bloch，1780年）和有钩绦虫（Batsch，1786年）。一个样本（0.7%）被鉴定为中线绦虫（Batsch，1786年）。所有样本的细粒棘球绦虫PCR检测均为阴性。综合粪便检查法和PCR结果，28%的犬感染了蠕虫（8%感染了绦虫）。应当强调的是，其中一只感染多房棘球绦虫的犬排出了带绦虫属类型的虫卵，并具有捕食啮齿动物的习性。本次调查首次在波兰的犬类中发现了多房棘球绦虫。
