Karamon Jacek, Samorek-Pieróg Małgorzata, Sroka Jacek, Bilska-Zając Ewa, Dąbrowska Joanna, Kochanowski Maciej, Różycki Mirosław, Zdybel Jolanta, Cencek Tomasz
Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, 24-100 Puławy, Poland.
Pathogens. 2021 Jul 6;10(7):853. doi: 10.3390/pathogens10070853.
The aim of this study is to confirm the presence and molecular identification of tapeworms in wolves from south-eastern Poland. An investigation was carried out on the intestines of 13 wolves from south-eastern Poland. The small intestines were divided into three equal segments. Each segment was separately examined using the sedimentation and counting technique (SCT). The detected tapeworms were isolated and identified by PCRs and sequencing ( and genes). Additionally, DNA isolated from the feces of wolves positive for tapeworms was examined with two diagnostic PCRs. The intestines of one wolf were positive for when assessed by SCT; the intestine was from a six-year-old male wolf killed in a communication accident. We detected 61 adult tapeworms: 42 in the anterior, 14 in the middle, and 5 in the posterior parts of the small intestine. The PCRs conducted for and produced specific products. A sequence comparison with the GenBank database showed similarity to the deposited (G5) sequences. An analysis of the available phylogenetic sequences showed very little variation within the species of (G5), and identity ranged from 99.10% to 100.00% in the case of and from 99.04% to 100.00% in the case of . One of the two diagnostic PCRs used and performed on the feces of -positive animals showed product specific for . This study showed the presence of adult tapeworms in wolves for the first time.
本研究的目的是确认波兰东南部狼体内绦虫的存在并进行分子鉴定。对来自波兰东南部的13只狼的肠道进行了调查。小肠被分成三个相等的部分。每个部分分别采用沉淀计数技术（SCT）进行检查。对检测到的绦虫进行分离，并通过聚合酶链式反应（PCR）和测序（针对 和 基因）进行鉴定。此外，对绦虫检测呈阳性的狼的粪便中分离出的DNA进行了两种诊断性PCR检测。通过SCT评估，有一只狼的肠道呈阳性；这只狼是一只6岁的雄性狼，死于交通事故。我们在小肠前部检测到61条成虫绦虫：42条，中部14条，后部5条。针对 和 进行的PCR产生了特异性产物。与GenBank数据库进行的序列比较显示与已存入的 （G5）序列相似。对现有系统发育序列的分析表明，在 （G5）物种内变异很小， 情况下的同一性范围为99.10%至100.00%， 情况下的同一性范围为99.04%至100.00%。对绦虫阳性动物粪便进行的两种诊断性PCR中的一种显示出针对 的特异性产物。本研究首次表明狼体内存在成虫绦虫。
