Wang Jun-Wei, Chen Xin-Yi, Hu Pei-Yang, Tan Ming-Ming, Tang Xiao-Gang, Huang Min-Cong, Lou Zhao-Huan
Emergency Department, People's Hospital of Tiantai County, Tiantai, Zhejiang 317200, P.R. China.
Safety Evaluation Center, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310007, P.R. China.
Exp Ther Med. 2016 Jun;11(6):2185-2192. doi: 10.3892/etm.2016.3244. Epub 2016 Apr 11.
Traditional treatments have a poor effect on alcoholic liver diseases. Linderae radix (LR), the dried root of (Sims) Kosterm., has been frequently used in traditional Chinese medicine for treating various diseases, and has been shown to exhibit a protective effect on liver injury. In the present study, LR extracts were made using various solvents, and then administrated to rats to establish a model of ethanol-induced liver injury. The study aimed to investigate the therapeutic effects and potential mechanism of LR extracts on acute alcoholic liver injury. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglycercide (TG), cholesterol (TC), methane dicarboxylic aldehyde (MDA) and superoxide dismutase (SOD) were determined using an automatic biochemistry analyzer. In addition, pathological examination was performed by hematoxylin-eosin staining. The levels of MDA and SOD, and the expression levels of nuclear factor (NF)-κB, tumor necrosis factor (TNF)-α and interleukin (IL)-1β in liver tissue were investigated immunohistochemically. The expression of cytochrome P450 2E1 (CYP2E1) mRNA was quantified by reverse transcription-quantitative polymerase chain reaction. The results indicated that LR extracts improved the histopathological status and decreased the serum levels of ALT, AST, TG, TC and MDA. Furthermore, the levels of MDA and inflammatory mediators (NF-κB, TNF-α and IL-1β) were decreased in liver tissues, and the overexpression of CYP2E1 mRNA induced by ethanol treatment. LR extracts exhibited a protective effect on alcoholic liver injury and the mechanism may be associated with the anti-inflammatory and anti-oxidative action.
传统治疗方法对酒精性肝病疗效不佳。乌药（Linderae radix，即乌药（Lindera aggregata (Sims) Kosterm.）的干燥根）在传统中药中常用于治疗多种疾病，且已显示出对肝损伤具有保护作用。在本研究中，使用各种溶剂制备乌药提取物，然后将其给予大鼠以建立乙醇诱导的肝损伤模型。该研究旨在探讨乌药提取物对急性酒精性肝损伤的治疗作用及潜在机制。使用自动生化分析仪测定血清中丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、甘油三酯（TG）、胆固醇（TC）、丙二醛（MDA）和超氧化物歧化酶（SOD）的水平。此外，通过苏木精-伊红染色进行病理检查。采用免疫组织化学方法研究肝组织中MDA和SOD的水平以及核因子（NF）-κB、肿瘤坏死因子（TNF）-α和白细胞介素（IL）-1β的表达水平。通过逆转录-定量聚合酶链反应对细胞色素P450 2E1（CYP2E1）mRNA的表达进行定量分析。结果表明，乌药提取物改善了组织病理学状况，并降低了血清中ALT、AST、TG、TC和MDA的水平。此外，肝组织中MDA和炎症介质（NF-κB、TNF-α和IL-1β）的水平降低，乙醇处理诱导的CYP2E1 mRNA过表达也得到改善。乌药提取物对酒精性肝损伤具有保护作用，其机制可能与抗炎和抗氧化作用有关。
