Fan Haoning, Shao Meng, Huang Shaohui, Liu Ying, Liu Jie, Wang Zhiyuan, Diao Jianxin, Liu Yuanliang, Tong L I, Fan Qin
Department of Molecular Biology, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
Department of Radiotherapy, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
Oncol Lett. 2016 Jun;11(6):3729-3734. doi: 10.3892/ol.2016.4438. Epub 2016 Apr 14.
Curcumin (Cur) exhibits radiosensitization effects to a variety of malignant tumors. The present study investigates the radiosensitizing effect of Cur on nasopharyngeal carcinoma (NPC) cells and whether its mechanism is associated with microRNA-593 (miR-593) and multidrug resistance gene 1 (MDR1). A clonogenic assay was performed to measure the radiosensitizing effect. The expression of miR-593 and MDR1 was analyzed by quantitative polymerase chain reaction (qPCR) or western blot assay. A transplanted tumor model was established to identify the radiosensitizing effect . A luciferase-based reporter was constructed to evaluate the effect of direct binding of miR-593 to the putative target site on the 3' UTR of MDR1. The clonogenic assay showed that Cur enhanced the radiosensitivity of cells. Cur (100 mg/kg) combined with 4 Gy irradiation inhibited the growth of a transplanted tumor model , resulting in the higher inhibition ratio compared with the radiotherapy-alone group. These results demonstrated that Cur had a radiosensitizing effect on NPC cells and ; Cur-mediated upregulation of miR-593 resulted in reduced MDR1 expression, which may promote radiosensitivity of NPC cells.
姜黄素（Cur）对多种恶性肿瘤具有放射增敏作用。本研究探讨Cur对鼻咽癌（NPC）细胞的放射增敏作用及其机制是否与微小RNA - 593（miR - 593）和多药耐药基因1（MDR1）相关。进行克隆形成试验以测量放射增敏效果。通过定量聚合酶链反应（qPCR）或蛋白质免疫印迹法分析miR - 593和MDR1的表达。建立移植瘤模型以确定放射增敏效果。构建基于荧光素酶的报告基因以评估miR - 593与MDR1 3'UTR上假定靶位点直接结合的作用。克隆形成试验表明Cur增强了细胞的放射敏感性。Cur（100 mg/kg）联合4 Gy照射抑制了移植瘤模型的生长，与单纯放疗组相比，抑制率更高。这些结果表明Cur对NPC细胞具有放射增敏作用；Cur介导的miR - 593上调导致MDR1表达降低，这可能促进NPC细胞的放射敏感性。
