Huang Jing, Guo Na, Li Yinghui, Sun Jutao, Hu Guanjun, Zhang Haipeng, Li Yanfei, Zhang Xing, Zhao Jinming, Xing Han, Qiu Lijuan
National Center for Soybean Improvement/National Key laboratory of Crop Genetics and Germplasm enhancement, Key laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)/Key Lab of Germplasm Utilization (MOA), Institute of Crop Science, Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China.
BMC Genet. 2016 Jun 18;17(1):85. doi: 10.1186/s12863-016-0383-4.
Phytophthora root and stem rot (PRR) caused by Phytophthora sojae is one of the most serious diseases affecting soybean (Glycine max (L.) Merr.) production all over the world. The most economical and environmentally-friendly way to control the disease is the exploration and utilization of resistant varieties.
We screened a soybean mini core collection composed of 224 germplasm accessions for resistance against eleven P. sojae isolates. Soybean accessions from the Southern and Huanghuai regions, especially the Hubei, Jiangsu, Sichuan and Fujian provinces, had the most varied and broadest spectrum of resistance. Based on gene postulation, Rps1b, Rps1c, Rps4, Rps7 and novel resistance genes were identified in resistant accessions. Consequently, association mapping of resistance to each isolate was performed with 1,645 single nucleotide polymorphism (SNP) markers. A total of 14 marker-trait associations for Phytophthora resistance were identified. Among them, four were located in known PRR resistance loci intervals, five were located in other disease resistance quantitative trait locus (QTL) regions, and five associations unmasked novel loci for PRR resistance. In addition, we also identified candidate genes related to resistance.
This is the first P. sojae resistance evaluation conducted using the Chinese soybean mini core collection, which is a representative sample of Chinese soybean cultivars. The resistance reaction analyses provided an excellent database of resistant resources and genetic variations for future breeding programs. The SNP markers associated with resistance will facilitate marker-assisted selection (MAS) in breeding programs for resistance to PRR, and the candidate genes may be useful for exploring the mechanism underlying P. sojae resistance.
由大豆疫霉引起的大豆根茎腐病是影响全球大豆（Glycine max (L.) Merr.）生产的最严重病害之一。控制该病最经济环保的方法是探索和利用抗病品种。
我们对由224份种质组成的大豆微型核心种质库进行了筛选，以检测其对11株大豆疫霉菌株的抗性。来自南方和黄淮地区的大豆种质，特别是湖北、江苏、四川和福建等省的种质，具有最多样化和最广泛的抗性谱。基于基因推导，在抗病种质中鉴定出了Rps1b、Rps1c、Rps4、Rps7和新的抗性基因。因此，利用1645个单核苷酸多态性（SNP）标记对每个菌株的抗性进行了关联分析。共鉴定出14个与疫霉抗性相关的标记-性状关联。其中，4个位于已知的大豆根茎腐病抗性基因座区间，5个位于其他抗病数量性状基因座（QTL）区域，5个关联揭示了新的大豆根茎腐病抗性基因座。此外，我们还鉴定了与抗性相关的候选基因。
这是首次利用中国大豆微型核心种质库进行大豆疫霉抗性评价，该种质库是中国大豆品种的代表性样本。抗性反应分析为未来育种计划提供了优良的抗性资源和遗传变异数据库。与抗性相关的SNP标记将有助于在大豆根茎腐病抗性育种计划中进行标记辅助选择（MAS），候选基因可能有助于探索大豆疫霉抗性的潜在机制。
