Chou Hsin-Yu, Lee Chelsea, Pan Jian-Liang, Wen Zhi-Hong, Huang Shu-Hung, Lan Chi-Wei John, Liu Wang-Ta, Hour Tzyh-Chyuan, Hseu You-Cheng, Hwang Byeong Hee, Cheng Kuo-Chen, Wang Hui-Min David
Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Division of Biochemistry and Molecular Biology, Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Int J Mol Sci. 2016 Jun 16;17(6):955. doi: 10.3390/ijms17060955.
Among many antioxidants that are used for the repairing of oxidative stress induced skin damages, we identified the enriched astaxanthin extract (EAE) from Haematococcus pluvialis as a viable ingredient. EAE was extracted from the red microalgae through supercritical fluid carbon dioxide extraction. To compare the effectiveness, EAE wastreated on human dermal fibroblasts with other components, phorbol 12-myristate 13-acetate (PMA), and doxycycline. With sirius red staining and quantitative real-time polymerase chain reaction (qRT-PCR), we found that PMA decreased the collagen concentration and production while overall the addition of doxycycline and EAE increased the collagen concentration in a trial experiments. EAE increased collagen contents through inhibited MMP1 and MMP3 mRNA expression and induced TIMP1, the antagonists of MMPs protein, gene expression. As for when tested for various proteins through western blotting, it was seen that the addition of EAE increased the expression of certain proteins that promote cell proliferation. Testing those previous solutions using growth factor assay, it was noticeable that EAE had a positive impact on cell proliferation and vascular endothelial growth factor (VEGF) than doxycycline, indicating that it was a better alternative treatment for collagen production. To sum up, the data confirmed the possible applications as medical cosmetology agentsand food supplements.
在众多用于修复氧化应激诱导的皮肤损伤的抗氧化剂中，我们确定从雨生红球藻中提取的富含虾青素的提取物（EAE）是一种可行的成分。EAE是通过超临界流体二氧化碳萃取法从红色微藻中提取的。为了比较其效果，将EAE与其他成分、佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）和强力霉素一起作用于人类皮肤成纤维细胞。通过天狼星红染色和定量实时聚合酶链反应（qRT-PCR），我们发现在一项试验中，PMA降低了胶原蛋白浓度和产量，而总体上添加强力霉素和EAE增加了胶原蛋白浓度。EAE通过抑制MMP1和MMP3 mRNA表达并诱导MMPs蛋白的拮抗剂TIMP1基因表达来增加胶原蛋白含量。当通过蛋白质印迹法检测各种蛋白质时，可以看到添加EAE增加了某些促进细胞增殖的蛋白质的表达。使用生长因子测定法测试之前的那些溶液时，值得注意的是，EAE对细胞增殖和血管内皮生长因子（VEGF）的影响比强力霉素更积极，表明它是胶原蛋白生成的更好替代治疗方法。总之，数据证实了其作为医学美容剂和食品补充剂的可能应用。
