Shin Jieun, Kim Jong-Eun, Pak Kum-Ju, Kang Jung Il, Kim Tae-Seok, Lee Sang-Yoon, Yeo Ik-Hyun, Park Jung Han Yoon, Kim Jong Hun, Kang Nam Joo, Lee Ki Won
Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Korea.
Research Institute of Biotechnology and Medical Converged Science, Dongguk University (Seoul),Goyang 10326, Korea.
Int J Mol Sci. 2017 Mar 22;18(3):682. doi: 10.3390/ijms18030682.
Soybean-derived isoflavones have been investigated for their preventative effects against UV-induced symptoms of skin damage including wrinkle formation and inflammation. Haematococcus pluvialis is a freshwater species of Chlorophyta that contains high concentrations of the natural carotenoid pigment astaxanthin. Astaxanthin is known to be involved in retinoic acid receptor (RAR) signaling and previously been associated with the inhibition of activator protein (AP)-1 dependent transcription. Based on previous studies, we hypothesized that a combination of soy extract (SE) and Haematococcus extract (HE) may prevent UVB-induced photoaging through specific signaling pathways, as measured by UVB-induced wrinkling on hairless mice skin and expression changes in human dermal fibroblasts (HDFs). The 1:2 ratio of SE and HE mixture (SHM) showed the optimal benefit in vivo. SHM was found to inhibit wrinkle formation via the downregulation of matrix metalloproteinase (MMP)-1 mRNA and protein expression. SHM also inhibited mitogen-activated protein kinase (MAPK) phosphorylation and the transactivation of AP-1 which plays an important role in regulating MMP expression. These results highlight the potential for SHM to be developed as a therapeutic agent to prevent UVB-induced skin wrinkling.
大豆衍生的异黄酮已被研究其对紫外线诱导的皮肤损伤症状（包括皱纹形成和炎症）的预防作用。雨生红球藻是绿藻门的一种淡水物种，含有高浓度的天然类胡萝卜素虾青素。已知虾青素参与视黄酸受体（RAR）信号传导，并且先前已与抑制激活蛋白（AP）-1依赖性转录相关联。基于先前的研究，我们假设大豆提取物（SE）和雨生红球藻提取物（HE）的组合可能通过特定的信号通路预防紫外线B诱导的光老化，这通过无毛小鼠皮肤上紫外线B诱导的皱纹以及人皮肤成纤维细胞（HDFs）中的表达变化来衡量。SE和HE混合物（SHM）的1:2比例在体内显示出最佳效果。发现SHM通过下调基质金属蛋白酶（MMP）-1 mRNA和蛋白质表达来抑制皱纹形成。SHM还抑制丝裂原活化蛋白激酶（MAPK）磷酸化以及在调节MMP表达中起重要作用的AP-1的反式激活。这些结果突出了SHM作为预防紫外线B诱导的皮肤皱纹的治疗剂的开发潜力。
