In Vu Van, Ntalamagka Nikoleta, O'Connor Wayne, Wang Tianfang, Powell Daniel, Cummins Scott F, Elizur Abigail
Centre of Genetics, Ecology and Physiology, University of the Sunshine Coast, Maroochydore, Queensland 4558, Australia; Northern National Broodstock Center for Mariculture, Research Institute for Aquaculture No. 1, Catba Islands, Haiphong, Vietnam.
Centre of Genetics, Ecology and Physiology, University of the Sunshine Coast, Maroochydore, Queensland 4558, Australia.
Peptides. 2016 Aug;82:109-119. doi: 10.1016/j.peptides.2016.06.007. Epub 2016 Jun 17.
The Sydney Rock Oyster, Saccostrea glomerata, is a socioeconomically important species in Australia, yet little is known about the molecular mechanism that regulates its reproduction. To address this gap, we have performed a combination of high throughput transcriptomic and peptidomic analysis, to identify genes and neuropeptides that are expressed in the key regulatory tissues of S. glomerata; the visceral ganglia and gonads. Neuropeptides are known to encompass a diverse class of peptide messengers that play functional roles in many aspects of an animal's life, including reproduction. Approximately 28 neuropeptide genes were identified, primarily within the visceral ganglia transcriptome, that encode precursor proteins containing numerous neuropeptides; some were confirmed through mass spectral peptidomics analysis of the visceral ganglia. Of those, 28 bioactive neuropeptides were synthesized, and then tested for their capacity to induce gonad development and spawning in S. glomerata. Egg laying hormone, gonadotropin-releasing hormone, APGWamide, buccalin, CCAP and LFRFamide were neuropeptides found to trigger spawning in ripe animals. Additional testing of APGWa and buccalin demonstrated their capacity to advance conditioning and gonadal maturation. In summary, our analysis of S. glomerata has identified neuropeptides that can influence the reproductive cycle of this species, specifically by accelerating gonadal maturation and triggering spawning. Other molluscan neuropeptides identified in this study will enable further research into understanding the neuroendocrinology of oysters, which may benefit their cultivation.
悉尼岩蚝（Saccostrea glomerata）是澳大利亚一种具有重要社会经济意义的物种，但对于调节其繁殖的分子机制却知之甚少。为了填补这一空白，我们进行了高通量转录组学和肽组学分析相结合的研究，以鉴定在悉尼岩蚝的关键调控组织——内脏神经节和性腺中表达的基因和神经肽。已知神经肽包含多种肽类信使，它们在动物生命的许多方面发挥功能作用，包括繁殖。我们主要在内脏神经节转录组中鉴定出约28个神经肽基因，这些基因编码含有众多神经肽的前体蛋白；其中一些通过内脏神经节的质谱肽组学分析得到了证实。在这些神经肽中，合成了28种生物活性神经肽，然后测试它们诱导悉尼岩蚝性腺发育和产卵的能力。产卵激素、促性腺激素释放激素、APGWamide、buccalin、CCAP和LFRFamide等神经肽被发现可触发成熟个体产卵。对APGWa和buccalin的进一步测试表明它们具有促进性腺成熟和性腺发育的能力。总之，我们对悉尼岩蚝的分析鉴定出了能够影响该物种繁殖周期的神经肽，特别是通过加速性腺成熟和触发产卵来实现。本研究中鉴定出的其他软体动物神经肽将有助于进一步研究牡蛎的神经内分泌学，这可能有益于它们的养殖。
