Noteborn H P, Weusten J J, Bartsch H, Bartsch C, Flehmig B, Ebels I, Salemink C A
Department of Organic Chemistry, University of Utrecht, The Netherlands.
J Pineal Res. 1989;6(4):385-96. doi: 10.1111/j.1600-079x.1989.tb00435.x.
A combination of gelfiltration and reverse-phase high performance liquid chromatography with postcolumn antitumour assay has been developed. A melatonin insensitive human melanoma cell strain was used to guide the purification of the antitumour effect of an ovine pineal aqueous extract (MW 1,000 to 10,000) that possessed the ability to decrease the hypophysiotropic activity of rat and mice hypothalami in vitro. This allows a specific identification of a pineal factor (MW 2,000 to 6,000) that inhibits the growth of human melanoma cells at a dose of 0.47 mg/ml medium. It was shown that the activity of this pineal compound differs from structures known to be present in the pineal, such as melatonin, pteridines, and beta-carbolines. There appears to be evidence for a peptidic nature of this pineal antitumour factor.
已开发出一种结合凝胶过滤和反相高效液相色谱以及柱后抗肿瘤测定的方法。使用对褪黑素不敏感的人黑色素瘤细胞系来指导纯化一种绵羊松果体水提取物（分子量1000至10000）的抗肿瘤作用，该提取物在体外具有降低大鼠和小鼠下丘脑促垂体活性的能力。这使得能够特异性鉴定出一种松果体因子（分子量2000至6000），其在培养基中浓度为0.47mg/ml时可抑制人黑色素瘤细胞的生长。结果表明，这种松果体化合物的活性不同于已知存在于松果体中的结构，如褪黑素、蝶啶和β-咔啉。似乎有证据表明这种松果体抗肿瘤因子具有肽的性质。
