Noteborn H P, Bartsch H, Bartsch C, Mans D R, Weusten J J, Flehmig B, Ebels I, Salemink C A
Department of Organic Chemistry, Utrecht University, The Netherlands.
J Neural Transm. 1988;73(2):135-55. doi: 10.1007/BF01243385.
An in vitro human melanoma cell assay was used to work up the partial purification of (a) low molecular weight (MW) substance(s) from aqueous extracts of ovine pineal tissue shown to contain a growth-inhibiting activity. A combination of paper chromatography, ion-exchange and reverse-phase high performance liquid chromatography with post-column antitumor assay has been developed. This allows a specific identification of an ovine pineal factor (MW less than 500) which inhibits the growth of human melanoma cells in vitro. The substance was partially purified to about 1,000 times as compared to the IC100-value of the starting material (retentate 5). The growth inhibition of human melanoma cells in culture was complete at a dose of 0.1 microgram/ml of purified pineal factor(s). It was demonstrated that the activity of this pineal compound differs from some substances known to be present in the pineal, such as melatonin, serotonin, peridines and beta-carbolines. The activity was not destroyed by treatment with proteolytic enzymes.
采用体外人黑素瘤细胞试验对从绵羊松果体组织水提取物中初步纯化出的一种低分子量物质进行研究，该提取物显示具有生长抑制活性。已开发出一种结合纸色谱法、离子交换和反相高效液相色谱法以及柱后抗肿瘤试验的方法。这使得能够特异性鉴定出一种绵羊松果体因子（分子量小于500），其在体外可抑制人黑素瘤细胞的生长。与起始材料（截留物5）的IC100值相比，该物质被部分纯化至约1000倍。纯化的松果体因子剂量为0.1微克/毫升时，可完全抑制培养的人黑素瘤细胞的生长。结果表明，这种松果体化合物的活性不同于一些已知存在于松果体中的物质，如褪黑素、血清素、哌啶和β-咔啉。该活性不会因用蛋白水解酶处理而被破坏。
