Ciesielczuk H, Jenkins C, Chattaway M, Doumith M, Hope R, Woodford N, Wareham D W
Antimicrobial Research Group, Centre for Immunology and Infectious Disease, Blizard Institute, Bart's and the London School of Medicine and Dentistry, Queen Mary, University of London, E1 2AT, London, UK.
Antimicrobial Resistance and Healthcare Associated Infections (AMRHAI) Reference Unit, Public Health England, Colindale, UK.
Eur J Clin Microbiol Infect Dis. 2016 Oct;35(10):1661-6. doi: 10.1007/s10096-016-2707-8. Epub 2016 Jun 22.
Extra-intestinal pathogenic Escherichia coli are a significant cause of urinary tract infection and bacteraemia within the UK. We sought to identify the serogroups of 658 E. coli isolates collected in the UK between January 2011 and March 2012, to better understand the ExPEC population and understand the relevance of serogroups in this pathotype. Isolates were typed and serogroup identified using established phenotypic and molecular methods. Sixty-two serogroups were identified; 54 among urinary isolates and 35 among bloodstream isolates. However, serogroups O25, O6, and O2 dominated both infection types. These serogroups were linked to the major ExPEC STs as follows: ST131-O25, ST73-O6, ST127-O6, and ST95-O2. The serogroup data from this study have increased our understanding of the ExPEC population in the UK, but also highlighted key ST-serogroup relationships within the major ExPEC clones. These data can be used to guide vaccine design and in the development of laboratory diagnostic tests targeting the ExPEC population.
肠外致病性大肠杆菌是英国尿路感染和菌血症的重要病因。我们试图鉴定2011年1月至2012年3月期间在英国收集的658株大肠杆菌分离株的血清群，以更好地了解肠外致病性大肠杆菌群体，并了解血清群在这种致病类型中的相关性。使用既定的表型和分子方法对分离株进行分型并鉴定血清群。共鉴定出62个血清群；尿液分离株中有54个，血流分离株中有35个。然而，血清群O25、O6和O2在两种感染类型中均占主导地位。这些血清群与主要的肠外致病性大肠杆菌序列类型相关如下：ST131-O25、ST73-O6、ST127-O6和ST95-O2。本研究的血清群数据增进了我们对英国肠外致病性大肠杆菌群体的了解，同时也突出了主要肠外致病性大肠杆菌克隆中关键的序列类型-血清群关系。这些数据可用于指导疫苗设计以及开发针对肠外致病性大肠杆菌群体的实验室诊断测试。
