Stoica Bogdan A, Bunescu Sabina, Neamtu Andrei, Bulgaru-Iliescu Diana, Foia Liliana, Botnariu Eosefina Gina
Department of Forensic Genetics and Serology, Institute of Legal Medicine, Str. Buna Vestire Nr. 4, 700455, IASI, Romania.
Department of Biochemistry, "Gr. T. Popa" University of Medicine and Pharmacy, Str. Universitatii Nr. 16, 700115, IASI, Romania.
J Forensic Sci. 2016 Sep;61(5):1331-6. doi: 10.1111/1556-4029.13141. Epub 2016 Jun 22.
The aim of this study was to develop chemical improvements to the original Weber protocol, in order to increase the intensity and time length of light emission and to eliminate false-positive reactions. The intensity and duration of light were measured on serial blood dilutions using a plate reader chemiluminometer. Blood stains of various concentrations were impregnated in pure cellulose, dried, and luminol solution was added with/without the potential enhancers. An in silico study was also conducted, aiming to demonstrate the enhancing mechanism of hemoglobin denaturation using 8 M urea. The luminol blood detection test revealed important improvements after urea pretreatment or in the presence of monochloro-triazinyl-β-cyclodextrin. This approach also eliminated the false-positive reaction from sodium hypochlorite. These improvements could provide a higher sensitivity under particular circumstances such as old or washed blood stains, leading to a better localization for further DNA typing and higher quality photographic analysis.
本研究的目的是对原始的韦伯协议进行化学改进，以增加发光强度和持续时间，并消除假阳性反应。使用酶标仪化学发光计对系列血液稀释液的光强度和持续时间进行测量。将不同浓度的血迹浸渍在纯纤维素中，干燥后，在有/无潜在增强剂的情况下加入鲁米诺溶液。还进行了一项计算机模拟研究，旨在证明使用8 M尿素时血红蛋白变性的增强机制。鲁米诺血液检测试验显示，经过尿素预处理或在存在一氯三嗪基-β-环糊精的情况下有重要改进。这种方法还消除了次氯酸钠引起的假阳性反应。这些改进可以在诸如陈旧或洗涤过的血迹等特殊情况下提供更高的灵敏度，从而为进一步的DNA分型提供更好的定位和更高质量的照片分析。
