Kim Joo-Young, Park Jung-Hyun, Kim Man Il, Lee Hye Hyeon, Kim Hye Lim, Jeong Kyu-Sik, Moon Sang-Ok, Kang Pil-Won, Park Ki-Won, Lee Yang-Han, Chun Byung-Won
Crime-scene DNA Section, National Forensic Service, Gwangju Institute, Gwangju, 57231, Republic of Korea.
Mibyeong Research Center, Korea Institute of Oriental Medicine, Daejeon, 34054, Republic of Korea.
Int J Legal Med. 2018 Jan;132(1):91-98. doi: 10.1007/s00414-017-1718-z. Epub 2017 Oct 30.
Blood stain evidence obtained from a violent crime scene provides decisive clues that can enable a case to be solved through forensic analyses such as genetic identification. However, collected samples usually contain a mixture of biological material from different sources, making genetic identification difficult. To address this issue, we developed an activatable aptamer sensor targeting 17β-estradiol for detection of female-specific blood in mixed samples. With the sensor, we were able to detect blood originating from females using a variable light source (495 nm). The sensor was especially sensitive to blood from young females (10-40 years) but not to blood from older females (≥ 50 years). Genomic DNA was extracted from the female blood specimens identified by this method and used for quantification and short tandem repeat genotyping. We confirmed that there was no fluorescence interference from the aptamer sensor. These results indicate that this novel aptamer sensor can be used to analyze evidentiary blood samples and thereby facilitate subsequent genetic identification.
从暴力犯罪现场获取的血迹证据提供了决定性线索，通过基因鉴定等法医分析手段能够破案。然而，采集的样本通常包含来自不同来源的生物物质混合物，这使得基因鉴定变得困难。为了解决这个问题，我们开发了一种靶向17β-雌二醇的可激活适体传感器，用于检测混合样本中的女性特异性血液。利用该传感器，我们能够使用可变光源（495纳米）检测出源自女性的血液。该传感器对年轻女性（10至40岁）的血液特别敏感，但对老年女性（≥50岁）的血液不敏感。从通过该方法鉴定出的女性血液样本中提取基因组DNA，并用于定量和短串联重复序列基因分型。我们证实适体传感器不存在荧光干扰。这些结果表明，这种新型适体传感器可用于分析血迹证据样本，从而便于后续的基因鉴定。
