Kali Arunava, Bhuvaneshwar Devaraj, Charles Pravin M V, Seetha Kunigal Srinivasaiah
Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Puducherry, India.
J Basic Clin Pharm. 2016 Jun;7(3):93-6. doi: 10.4103/0976-0105.183265.
The role of natural bioactive substances in treating infections has been rediscovered as bacterial resistance become common to most of the antibiotics. Curcumin is a bioactive substance from turmeric. Owing to antimicrobial properties, its prospect as an antibacterial agent is currently under focus.
We have evaluated the in vitro synergy of curcumin with antibiotics against sixty biofilm producing bacterial isolates. Congo red agar method was used to identify the biofilm producing isolates. Curcumin minimum inhibitory concentration (MIC) was determined by agar dilution method. Its antibiotic synergy was identified by the increase in disc diffusion zone size on Mueller-Hinton agar with 32 mg/L curcumin.
The mean MICs of curcumin against Gram-positive and Gram-negative isolates were 126.9 mg/L and 117.4 mg/L, respectively. Maximum synergy was observed with ciprofloxacin among Gram-positive and amikacin, gentamicin, and cefepime among Gram-negative isolates.
Curcumin per se as well as in combination with other antibiotics has a demonstrable antibacterial action against biofilm producing bacterial isolates. It may have a beneficial role in supplementing antibiotic therapy.
随着细菌对大多数抗生素普遍产生耐药性，天然生物活性物质在治疗感染中的作用被重新发现。姜黄素是姜黄中的一种生物活性物质。由于其抗菌特性，它作为一种抗菌剂的前景目前受到关注。
我们评估了姜黄素与抗生素对60株产生物膜细菌分离株的体外协同作用。采用刚果红琼脂法鉴定产生物膜的分离株。通过琼脂稀释法测定姜黄素的最低抑菌浓度（MIC）。通过在含32mg/L姜黄素的穆勒-欣顿琼脂上纸片扩散法抑菌圈大小的增加来确定其与抗生素的协同作用。
姜黄素对革兰氏阳性菌和革兰氏阴性菌分离株的平均MIC分别为126.9mg/L和117.4mg/L。在革兰氏阳性菌中，与环丙沙星协同作用最强；在革兰氏阴性菌中，与阿米卡星、庆大霉素和头孢吡肟协同作用最强。
姜黄素本身以及与其他抗生素联合使用时，对产生物膜细菌分离株具有明显的抗菌作用。它在补充抗生素治疗方面可能具有有益作用。
