Carneiro Bruno M, Batista Mariana N, Braga Ana Cláudia S, Nogueira Maurício L, Rahal Paula
Laboratory of Genomic Studies, Sao Paulo State University - UNESP, São José do Rio Preto, SP, Brazil.
Laboratory of Genomic Studies, Sao Paulo State University - UNESP, São José do Rio Preto, SP, Brazil.
Virology. 2016 Sep;496:215-218. doi: 10.1016/j.virol.2016.06.012. Epub 2016 Jun 23.
During ZIKV the outbreak in Brazil it was observed an increase of almost 20 times the number of reported cases of microcephaly in newborn babies. There is no vaccine or approved drug available for the treatment and prevention of infections by this virus. EGCG, a polyphenol present in green tea has been shown to have an antiviral activity for many viruses. In view of the need for the development of a drug against a Brazilian strain of ZIKV, we assessed the effect of EGCG on ZIKV entry in Vero E6 cells. The drug was capable of inhibiting the virus entry by at least 1-log (>90%) at higher concentrations (>100μM). The pre-treatment of cells with EGCG did not show any effect on virus attachment. This is the first study to demonstrate the effect of EGCG on ZIKV indicating that this drug might be possibility to be used for prevention of Zika virus infections.
在巴西寨卡病毒疫情爆发期间，观察到新生儿小头畸形报告病例数增加了近20倍。目前没有疫苗或获批药物可用于治疗和预防这种病毒感染。表没食子儿茶素没食子酸酯（EGCG）是绿茶中的一种多酚，已显示对多种病毒具有抗病毒活性。鉴于需要开发针对巴西寨卡病毒株的药物，我们评估了EGCG对寨卡病毒进入非洲绿猴肾细胞（Vero E6）的影响。在较高浓度（>100μM）下，该药物能够至少1个对数级（>90%）抑制病毒进入。用EGCG对细胞进行预处理对病毒附着没有任何影响。这是第一项证明EGCG对寨卡病毒有作用的研究，表明这种药物可能有用于预防寨卡病毒感染的可能性。
