Department of Biological Science, Seton Hall University, South Orange, NJ 07079, USA.
Food Chem Toxicol. 2013 Feb;52:207-15. doi: 10.1016/j.fct.2012.11.006. Epub 2012 Nov 23.
Green tea polyphenol epigallocatechin gallate (EGCG) is a strong antioxidant that has previously been shown to reduce the number of plaques in HIV-infected cultured cells. Modified EGCG, palmitoyl-EGCG (p-EGCG), is of interest as a topical antiviral agent for herpes simplex virus (HSV-1) infections. This study evaluated the effect of p-EGCG on HSV-infected Vero cells. Results of cell viability and cell proliferation assays indicate that p-EGCG is not toxic to cultured Vero cells and show that modification of the green tea polyphenol epigallocatechin gallate (EGCG) with palmitate increases the effectiveness of EGCG as an antiviral agent. Furthermore, p-EGCG is a more potent inhibitor of herpes simplex virus 1 (HSV-1) than EGCG and can be topically applied to skin, one of the primary tissues infected by HSV. Viral binding assay, plaque forming assay, PCR, real-time PCR, and fluorescence microscopy were used to demonstrate that p-EGCG concentrations of 50 μM and higher block the production of infectious HSV-1 particles. p-EGCG was found to inhibit HSV-1 adsorption to Vero cells. Thus, p-EGCG may provide a novel treatment for HSV-1 infections.
绿茶多酚表没食子儿茶素没食子酸酯（EGCG）是一种很强的抗氧化剂，先前已被证明可减少感染 HIV 的培养细胞中的斑块数量。棕榈酰化 EGCG（p-EGCG）是一种有前途的单纯疱疹病毒（HSV-1）感染的局部抗病毒药物。本研究评估了 p-EGCG 对感染 HSV 的vero 细胞的影响。细胞活力和细胞增殖试验的结果表明，p-EGCG 对培养的 vero 细胞没有毒性，并表明用棕榈酸修饰绿茶多酚表没食子儿茶素没食子酸酯（EGCG）增加了 EGCG 作为抗病毒剂的有效性。此外，p-EGCG 是一种比 EGCG 更有效的单纯疱疹病毒 1（HSV-1）抑制剂，可局部应用于皮肤，这是 HSV 感染的主要组织之一。病毒结合试验、空斑形成试验、PCR、实时 PCR 和荧光显微镜用于证明 50 μM 及更高浓度的 p-EGCG 可阻断感染性 HSV-1 颗粒的产生。发现 p-EGCG 可抑制 HSV-1 吸附到 vero 细胞。因此，p-EGCG 可能为 HSV-1 感染提供一种新的治疗方法。
