Shukla Rajesh, Kashaw Sushil K, Jain Alok Pal, Lodhi Santram
Department of Pharmaceutical Science, Suresh Gyan Vihar University, Jaipur, RJ, India.
Department of Pharmaceutical Sciences, Dr. H. S. Gour Central University, Sagar, MP, 470003, India.
Int J Biol Macromol. 2016 Oct;91:1110-9. doi: 10.1016/j.ijbiomac.2016.06.075. Epub 2016 Jun 23.
The Apigenin (APN) was isolated from ethanolic extract of M. alba leaves and screened by in-vivo wound models (Diabetic and Dead space) in rats. Apigenin loaded hydrogel (HGs) was prepared using gellan gum-chitosan (GGCH) with PEG as a cross linker and characterized for various parameter like AFM, swelling property, entrapment efficiency and drug release. Further performance of hydrogel was evaluated by wound healing activity tested against wound contraction, collagen content, dried granuloma weights and antioxidant activity. The percent entrapment efficiency of optimized hydrogel found to be 87.15±1.20. APN loaded GGCH-HGs were able to release 96.11% APN in 24h. The level of superoxide dismutase (SOD) and catalase were found increased significantly in granuloma tissue of APN treated group. APN GGCH-HGs found higher wound healing effect in diabetic as well as normal wound tissues with significant antioxidant activity. Results proven the utility of prepared hydrogel (APN loaded GGCH-HGs) seems to be highly suitable for wound healing due to its unique properties of biocompatibility, biodegradability, moist nature and antioxidant effectiveness.
芹菜素（APN）从白兰花叶乙醇提取物中分离出来，并通过大鼠体内伤口模型（糖尿病和死腔模型）进行筛选。以结冷胶-壳聚糖（GGCH）为原料，聚乙二醇为交联剂制备了负载芹菜素的水凝胶（HGs），并对其进行了原子力显微镜、溶胀性能、包封率和药物释放等各项参数的表征。通过对伤口收缩、胶原蛋白含量、干肉芽肿重量和抗氧化活性等指标的检测，进一步评估了水凝胶的伤口愈合活性。优化后的水凝胶包封率为87.15±1.20%。负载APN的GGCH-HGs在24小时内能够释放96.11%的APN。在APN治疗组的肉芽肿组织中，超氧化物歧化酶（SOD）和过氧化氢酶水平显著升高。APN GGCH-HGs在糖尿病伤口组织和正常伤口组织中均表现出较高的伤口愈合效果，并具有显著的抗氧化活性。结果证明，所制备的水凝胶（负载APN的GGCH-HGs）因其具有生物相容性、生物可降解性、湿润性和抗氧化有效性等独特性能，似乎非常适合用于伤口愈合。
