在低氧条件下从人包皮成纤维细胞生成诱导多能干细胞系iPSC-FH2.1。
Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts.
作者信息
Questa María, Romorini Leonardo, Blüguermann Carolina, Solari Claudia María, Neiman Gabriel, Luzzani Carlos, Scassa María Élida, Sevlever Gustavo Emilio, Guberman Alejandra Sonia, Miriuka Santiago Gabriel
机构信息
LIAN-CONICET, FLENI, Ruta 9 Km 52.5, Belén de Escobar B1625XAF, Buenos Aires, Argentina; Laboratorio de Regulación de la Expresión Génica en Células Madre, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN, CONICET-UBA, Intendente Güiraldes 2160, Ciudad Universitaria, Capital Federal, C1428EGA, Buenos Aires, Argentina.
LIAN-CONICET, FLENI, Ruta 9 Km 52.5, Belén de Escobar B1625XAF, Buenos Aires, Argentina; Carrera de Investigador, CONICET, Argentina.
出版信息
Stem Cell Res. 2016 Mar;16(2):300-3. doi: 10.1016/j.scr.2015.12.026. Epub 2016 Jan 4.
Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls.
在5%氧气培养条件下，使用表达OCT4、SOX2、c-MYC和KLF4的EF1a-hSTEMCCA-loxP载体，利用人包皮成纤维细胞生成了诱导多能干细胞系iPSC-FH2.1。在常氧和低氧条件下，均证实了其干性以及体内和体外的多能性。使用人胚胎干细胞（hESC）系WA-09和重编程的成纤维细胞原代培养物HFF-FM作为对照。
Zotero 插件