• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

在低氧条件下从人包皮成纤维细胞生成诱导多能干细胞系iPSC-FH2.1。

Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts.

作者信息

Questa María, Romorini Leonardo, Blüguermann Carolina, Solari Claudia María, Neiman Gabriel, Luzzani Carlos, Scassa María Élida, Sevlever Gustavo Emilio, Guberman Alejandra Sonia, Miriuka Santiago Gabriel

机构信息

LIAN-CONICET, FLENI, Ruta 9 Km 52.5, Belén de Escobar B1625XAF, Buenos Aires, Argentina; Laboratorio de Regulación de la Expresión Génica en Células Madre, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN, CONICET-UBA, Intendente Güiraldes 2160, Ciudad Universitaria, Capital Federal, C1428EGA, Buenos Aires, Argentina.

LIAN-CONICET, FLENI, Ruta 9 Km 52.5, Belén de Escobar B1625XAF, Buenos Aires, Argentina; Carrera de Investigador, CONICET, Argentina.

出版信息

Stem Cell Res. 2016 Mar;16(2):300-3. doi: 10.1016/j.scr.2015.12.026. Epub 2016 Jan 4.

DOI:10.1016/j.scr.2015.12.026
PMID:27345989
Abstract

Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls.

摘要

在5%氧气培养条件下，使用表达OCT4、SOX2、c-MYC和KLF4的EF1a-hSTEMCCA-loxP载体，利用人包皮成纤维细胞生成了诱导多能干细胞系iPSC-FH2.1。在常氧和低氧条件下，均证实了其干性以及体内和体外的多能性。使用人胚胎干细胞（hESC）系WA-09和重编程的成纤维细胞原代培养物HFF-FM作为对照。

相似文献

1
Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts.在低氧条件下从人包皮成纤维细胞生成诱导多能干细胞系iPSC-FH2.1。
Stem Cell Res. 2016 Mar;16(2):300-3. doi: 10.1016/j.scr.2015.12.026. Epub 2016 Jan 4.
2
Generation of induced pluripotent stem cell line from foreskin fibroblasts.从包皮成纤维细胞中诱导生成多能干细胞系。
Stem Cell Res. 2016 Nov;17(3):572-575. doi: 10.1016/j.scr.2016.09.014. Epub 2016 Sep 15.
3
Differentiation of human foreskin fibroblast-derived induced pluripotent stem cells into hepatocyte-like cells.人包皮成纤维细胞来源的诱导多能干细胞向肝细胞样细胞的分化
Cell Biochem Funct. 2016 Oct;34(7):475-482. doi: 10.1002/cbf.3210. Epub 2016 Aug 29.
4
Episomal-based generation of an iPS cell line from human fetal foreskin fibroblasts.基于游离型载体从人胎儿包皮成纤维细胞生成诱导多能干细胞系
Stem Cell Res. 2016 Jan;16(1):67-9. doi: 10.1016/j.scr.2015.12.009. Epub 2015 Dec 17.
5
Generation of integration free human iPSC line SORMi002-A from neonatal foreskin fibroblasts.从新生儿包皮成纤维细胞中生成无整合的人诱导多能干细胞系SORMi002-A。
Stem Cell Res. 2019 Oct;40:101534. doi: 10.1016/j.scr.2019.101534. Epub 2019 Aug 9.
6
Generation and characterization of two iPSC lines from human epicardium-derived cells.源自人心脏外膜细胞的两条诱导多能干细胞系的产生与鉴定
Stem Cell Res. 2017 Apr;20:50-53. doi: 10.1016/j.scr.2017.02.007. Epub 2017 Feb 24.
7
Characterization of iPSCs derived from dermal fibroblasts from a healthy 19year old female.对源自一名19岁健康女性真皮成纤维细胞的诱导多能干细胞的特性分析。
Stem Cell Res. 2016 Nov;17(3):597-599. doi: 10.1016/j.scr.2016.10.002. Epub 2016 Oct 19.
8
A feeder- and xeno-free human induced pluripotent stem cell line obtained from primary human dermal fibroblasts with epigenetic repression of reprogramming factors expression: GPCCi001-A.一种从原代人皮肤成纤维细胞获得的无饲养层和无外源物的人诱导多能干细胞系，具有重编程因子表达的表观遗传抑制：GPCCi001-A。
Stem Cell Res. 2017 Apr;20:34-37. doi: 10.1016/j.scr.2017.02.004. Epub 2017 Feb 21.
9
Efficient induction of pluripotent stem cells from menstrual blood.从月经血中高效诱导多能干细胞。
Stem Cells Dev. 2013 Apr 1;22(7):1147-58. doi: 10.1089/scd.2012.0428. Epub 2012 Dec 21.
10
Generation of iPSC line HEL24.3 from human neonatal foreskin fibroblasts.从人新生儿包皮成纤维细胞中生成诱导多能干细胞系HEL24.3。
Stem Cell Res. 2015 Jul;15(1):266-8. doi: 10.1016/j.scr.2015.05.012. Epub 2015 Jun 2.

引用本文的文献

1
Bioprinted research models of urological malignancy.泌尿外科恶性肿瘤的生物打印研究模型
Exploration (Beijing). 2024 Feb 20;4(4):20230126. doi: 10.1002/EXP.20230126. eCollection 2024 Aug.
2
CDK5 Deficiency Does not Impair Neuronal Differentiation of Human Induced Pluripotent Stem Cells but Affects Neurite Outgrowth.细胞周期蛋白依赖性激酶5缺乏并不损害人类诱导多能干细胞的神经元分化，但会影响神经突生长。
Mol Neurobiol. 2025 Jan;62(1):918-934. doi: 10.1007/s12035-024-04325-y. Epub 2024 Jun 28.
3
Role of hydroxymethylglutharyl-coenzyme A reductase in the induction of stem-like states in breast cancer.
羟甲基戊二酰辅酶 A 还原酶在乳腺癌诱导干细胞样状态中的作用。
J Cancer Res Clin Oncol. 2024 Feb 28;150(2):106. doi: 10.1007/s00432-024-05607-7.
4
Acute severe hypoxia induces apoptosis of human pluripotent stem cells by a HIF-1α and P53 independent mechanism.急性严重缺氧通过一种 HIF-1α 和 P53 非依赖的机制诱导人多能干细胞凋亡。
Sci Rep. 2022 Nov 5;12(1):18803. doi: 10.1038/s41598-022-23650-7.
5
Systemic proteomics and miRNA profile analysis of exosomes derived from human pluripotent stem cells.人多能干细胞来源的外泌体的系统蛋白质组学和 miRNA 谱分析。
Stem Cell Res Ther. 2022 Sep 5;13(1):449. doi: 10.1186/s13287-022-03142-1.
6
Stable expression of a truncated TLX variant drives differentiation of induced pluripotent stem cells into self-renewing neural stem cells for production of extracellular vesicles.稳定表达截断的 TLX 变体可驱动诱导多能干细胞分化为自我更新的神经干细胞，从而产生细胞外囊泡。
Stem Cell Res Ther. 2022 Sep 2;13(1):436. doi: 10.1186/s13287-022-03131-4.
7
Protocol for morphometric analysis of neurons derived from human pluripotent stem cells.人类多能干细胞来源神经元的形态计量分析方案。
STAR Protoc. 2022 Jun 18;3(3):101487. doi: 10.1016/j.xpro.2022.101487. eCollection 2022 Sep 16.
8
Single cell transfection of human-induced pluripotent stem cells using a droplet-based microfluidic system.使用基于液滴的微流控系统对人诱导多能干细胞进行单细胞转染。
R Soc Open Sci. 2022 Jan 12;9(1):211510. doi: 10.1098/rsos.211510. eCollection 2022 Jan.
9
celldeath: A tool for detection of cell death in transmitted light microscopy images by deep learning-based visual recognition.细胞死亡：基于深度学习的视觉识别在明场显微镜图像中检测细胞死亡的工具。
PLoS One. 2021 Jun 24;16(6):e0253666. doi: 10.1371/journal.pone.0253666. eCollection 2021.
10
Chemical hypoxia induces apoptosis of human pluripotent stem cells by a NOXA-mediated HIF-1α and HIF-2α independent mechanism.化学缺氧通过 NOXA 介导的 HIF-1α 和 HIF-2α 非依赖机制诱导人多能干细胞凋亡。
Sci Rep. 2020 Nov 26;10(1):20653. doi: 10.1038/s41598-020-77792-7.