基于游离质粒从胎儿股骨来源的人间充质基质细胞生成诱导多能干细胞。
Episomal plasmid-based generation of induced pluripotent stem cells from fetal femur-derived human mesenchymal stromal cells.
作者信息
Megges Matthias, Oreffo Richard O C, Adjaye James
机构信息
Institute for Stem Cell Research and Regenerative Medicine, Heinrich Heine University Duesseldorf, Moorenstr. 5, 40225 Duesseldorf, Germany; Max Planck Institute for Molecular Genetics, Ihnestraße 63-73, 14195 Berlin, Germany; Department of Biology, Chemistry and Pharmacy, Institute of Chemistry and Biochemistry, Freie Universität Berlin, Thielallee 63, 14195, Berlin, Germany.
Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton SO16 6YD, UK.
出版信息
Stem Cell Res. 2016 Jan;16(1):128-32. doi: 10.1016/j.scr.2015.12.013. Epub 2015 Dec 28.
Human bone mesenchymal stromal cells derived from fetal femur 55 days post-conception were reprogrammed to induced pluripotent stem cells using episomal plasmid-based expression of OCT4, SOX2, NANOG, LIN28, SV40LT, KLF4 and c-MYC and supplemented with the following pathway inhibitors - TGFβ receptor inhibitor (A-83-01), MEK inhibitor (PD325901), GSK3β inhibitor (CHIR99021) and ROCK inhibitor (HA-100). Successful induction of pluripotency in two iPS-cell lines was demonstrated in vitro and by the Pluritest.
利用基于游离型质粒表达的OCT4、SOX2、NANOG、LIN28、SV40LT、KLF4和c-MYC，将源自受孕后55天胎儿股骨的人骨髓间充质基质细胞重编程为诱导多能干细胞，并补充以下信号通路抑制剂——TGFβ受体抑制剂（A-83-01）、MEK抑制剂（PD325901）、GSK3β抑制剂（CHIR99021）和ROCK抑制剂（HA-100）。通过体外实验和Pluritest检测，证实了两个诱导多能干细胞系成功诱导出多能性。
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