Kickler T S, Bell W, Drew H, Pall D
Department of Laboratory Medicine, Johns Hopkins Hospital, Baltimore, Maryland.
Transfusion. 1989 Jun;29(5):411-4. doi: 10.1046/j.1537-2995.1989.29589284140.x.
Removal of white cells (WBCs) from platelets may reduce alloimmunization to WBC antigens, prevent febrile reactions, and improve platelet increments in multiply transfused patients receiving HLA-matched platelets. A new surface-modified fibrous polyester filter was evaluated; it requires no special processing of pooled platelet concentrates and can be used at the patient's bedside. The studies were designed to measure WBC removal, platelet function, in vitro platelet recovery, and in vivo platelet survival. WBC mean removal was 99.8 percent +/- 0.56 (n = 37) when a pool similar in volume to 6 platelet concentrates was tested. The mean number of residual WBCs after filtration was 5.6 x 10(5). In vitro mean platelet recovery was 86.9 percent for a pool size of 6 units (n = 37). Clot retraction and platelet aggregation were unaffected by filtration. Survival studies of 111Indium-labeled platelets done with filtered autologous platelets showed no reduction in the normally expected survival. These studies indicated that the filter efficiently removes WBCs without substantially decreasing platelet number, survival, or function. This device offers the potential of considerably improving platelet transfusion therapy.
从血小板中去除白细胞（WBC）可减少对WBC抗原的同种免疫，预防发热反应，并提高接受HLA匹配血小板多次输血患者的血小板增量。对一种新型表面改性纤维聚酯过滤器进行了评估；它不需要对混合血小板浓缩物进行特殊处理，并且可以在患者床边使用。这些研究旨在测量白细胞去除率、血小板功能、体外血小板回收率和体内血小板存活率。当测试一个体积与6个血小板浓缩物相似的混合样本时，白细胞平均去除率为99.8%±0.56（n = 37）。过滤后残余白细胞的平均数量为5.6×10⁵。对于6单位的混合样本，体外平均血小板回收率为86.9%（n = 37）。凝血块回缩和血小板聚集不受过滤影响。对用过滤后的自体血小板进行的¹¹¹铟标记血小板存活研究表明，其正常预期存活率没有降低。这些研究表明，该过滤器能有效去除白细胞，而不会大幅减少血小板数量、存活率或功能。这种装置具有显著改善血小板输血治疗的潜力。
