Jivrajani Mehul, Ravat Nirav, Anandjiwala Sheetal, Nivsarkar Manish
Department of Pharmacology and Toxicology, B. V. Patel Pharmaceutical Education and Research Development (PERD) Centre, Sarkhej-Gandhinagar Highway, Thaltej, Ahmedabad, Gujarat 380 054, India.
Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Ahmedabad, Gujarat 380054, India.
Int Sch Res Notices. 2014 Oct 29;2014:241946. doi: 10.1155/2014/241946. eCollection 2014.
The anti-implantation potential of different fractions of Vitex negundo Linn leaf extract was evaluated in female Swiss Albino mice. Animals from different groups were dosed orally either with 0.2% agar (vehicle) or with fractions of V. negundo leaf extract (n-hexane, chloroform, n-butanol, and remnant fractions) at 10:00 a.m., from day 1 to day 6 of pregnancy. The pregnant females from each group were sacrificed on different days of pregnancy (n = 6), and uterus was excised and used for estimation of lipid peroxidation and assay of superoxide dismutase activity as a marker for blastocyst implantation. Animals treated with n-hexane fraction showed altered level of superoxide anion radical and superoxide dismutase activity as compared to control animals. The probable mechanism by which this extract exhibits inhibition of blastocyst implantation is through the anti-inflammatory and antiestrogenic potential.
在雌性瑞士白化小鼠中评估了黄荆叶提取物不同馏分的抗着床潜力。从妊娠第1天到第6天上午10点，给不同组的动物口服0.2%琼脂（赋形剂）或黄荆叶提取物的馏分（正己烷、氯仿、正丁醇和残余馏分）。每组的妊娠雌性在妊娠的不同天数处死后（n = 6），切除子宫并用于估计脂质过氧化和测定超氧化物歧化酶活性，作为胚泡着床的标志物。与对照动物相比，用正己烷馏分处理的动物超氧阴离子自由基水平和超氧化物歧化酶活性发生了改变。该提取物表现出抑制胚泡着床的可能机制是通过抗炎和抗雌激素潜力。
