Saijai Sakuntala, Ando Akinori, Inukai Ryuya, Shinohara Makoto, Ogawa Jun
a Division of Applied Life Sciences , Graduate School of Agriculture, Kyoto University , Kyoto , Japan.
b Research Unit for the Physiological Chemistry , Kyoto University , Kyoto , Japan.
Biosci Biotechnol Biochem. 2016 Nov;80(11):2247-2254. doi: 10.1080/09168451.2016.1200459. Epub 2016 Jun 27.
Nitrifying microbial consortia were enriched from bark compost in a water system by regulating the amounts of organic nitrogen compounds and by controlling the aeration conditions with addition of CaCO for maintaining suitable pH. Repeated enrichment showed reproducible mineralization of organic nitrogen via the conversion of ammonium ions ( ) and nitrite ions ( ) into nitrate ions ( ). The change in microbial composition during the enrichment was investigated by PCR-DGGE analysis with a focus on prokaryote, ammonia-oxidizing bacteria, nitrite-oxidizing bacteria, and eukaryote cell types. The microbial transition had a simple profile and showed clear relation to nitrogen ions transition. Nitrosomonas and Nitrobacter were mainly detected during and oxidation, respectively. These results revealing representative microorganisms acting in each ammonification and nitrification stages will be valuable for the development of artificial simple microbial consortia for organic hydroponics that consisted of identified heterotrophs and autotrophic nitrifying bacteria.
通过调节有机氮化合物的量，并通过添加碳酸钙以维持合适的pH值来控制曝气条件，在水系统中从树皮堆肥中富集硝化微生物群落。重复富集表明，通过将铵离子（ ）和亚硝酸根离子（ ）转化为硝酸根离子（ ），有机氮可进行可重复的矿化。通过PCR-DGGE分析研究了富集过程中微生物组成的变化，重点关注原核生物、氨氧化细菌、亚硝酸氧化细菌和真核细胞类型。微生物转变具有简单的特征，并且与氮离子转变显示出明显的关系。分别在 氧化和 氧化过程中主要检测到亚硝化单胞菌和硝化杆菌。这些揭示了在每个氨化和硝化阶段起作用的代表性微生物的结果，对于开发由已鉴定的异养生物和自养硝化细菌组成的用于有机水培的人工简单微生物群落具有重要价值。
