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新发现的 Atlastin 家族成员 BmAtlastin-n 对家蚕杆状病毒（BmNPV）具有抗病毒作用。

A newly discovered member of the Atlastin family, BmAtlastin-n, has an antiviral effect against BmNPV in Bombyx mori.

机构信息

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.

Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, China.

出版信息

Sci Rep. 2016 Jun 29;6:28946. doi: 10.1038/srep28946.

DOI:10.1038/srep28946
PMID:27353084
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4926086/
Abstract

Atlastin is a member of the dynamin protein superfamily and it can mediate homotypic fusion of endoplasmic reticulum (ER) membranes, which is required for many biological processes. In this study, a new Atlastin homologous protein, BmAtlastin-n, was characterized in silkworms and was found to contain an N-terminal conserved GTPase domain and a coiled-coil middle domain. BmAtlastin-n is localized in the cytoplasm and enriched in silkworm midgut. Results also showed that overexpression of BmAtlastin-n in BmN-SWU1 cells could enhance resistance to BmNPV. To better confirm its antiviral effect, microRNA was used to knock down the expression of BmAtlastin-n in BmE-SWU1 cells with inducing the reproduction of BmNPV. A transgenic expression vector of BmAtlastin-n was constructed and introduced to silkworm embryos by microinjection. The transgenic silkworm also showed considerable antiviral capacity. In conclusion, these findings demonstrate that BmAtlastin-n plays an important role in BmNPV defense. More importantly, the current study may provide a new clue for Atlastin research.

摘要

Atlastin 是动力蛋白超家族的成员，它可以介导内质网 (ER) 膜的同源融合，这是许多生物过程所必需的。在本研究中，在蚕中鉴定了一种新的 Atlastin 同源蛋白 BmAtlastin-n，它含有一个 N 端保守的 GTPase 结构域和一个卷曲螺旋中间结构域。BmAtlastin-n 定位于细胞质中，在蚕中肠中富集。结果还表明，在 BmN-SWU1 细胞中过表达 BmAtlastin-n 可以增强对 BmNPV 的抗性。为了更好地确认其抗病毒作用，用 microRNA 敲低 BmE-SWU1 细胞中 BmAtlastin-n 的表达，诱导 BmNPV 的繁殖。构建了 BmAtlastin-n 的转基因表达载体，并通过显微注射将其导入蚕胚胎。转基因蚕也表现出相当大的抗病毒能力。总之，这些发现表明 BmAtlastin-n 在 BmNPV 防御中发挥重要作用。更重要的是，本研究可能为 Atlastin 的研究提供新的线索。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c794/4926086/9b5b28b23d87/srep28946-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c794/4926086/935223bebe97/srep28946-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c794/4926086/793d6c45c41d/srep28946-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c794/4926086/b3afaffe726e/srep28946-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c794/4926086/aa5e2f057f71/srep28946-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c794/4926086/d4163dca5a58/srep28946-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c794/4926086/9b5b28b23d87/srep28946-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c794/4926086/935223bebe97/srep28946-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c794/4926086/793d6c45c41d/srep28946-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c794/4926086/b3afaffe726e/srep28946-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c794/4926086/aa5e2f057f71/srep28946-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c794/4926086/d4163dca5a58/srep28946-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c794/4926086/9b5b28b23d87/srep28946-f6.jpg

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