通过荧光定位法观察DNA折纸与溶酶体之间的细胞内相互作用。
Observation of intracellular interactions between DNA origami and lysosomes by the fluorescence localization method.
作者信息
Fu Meifang, Dai Luru, Jiang Qiao, Tang Yunqing, Zhang Xiaoming, Ding Baoquan, Li Junbai
机构信息
Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Lab of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
出版信息
Chem Commun (Camb). 2016 Jul 28;52(59):9240-2. doi: 10.1039/c6cc00484a. Epub 2016 Jun 29.
We obtained the fluorescence localization images of tube DNA origami nanostructures in NIH 3T3 cells for the first time. The fluorescence localization images of tube DNA origami nanostructures and TIRF images of lysosomes were combined and they revealed the detailed interactions between the two structures. Quantitative analysis illustrated that the tube origami can be captured as well as degraded by lysosomes with time.
我们首次获得了管状DNA折纸纳米结构在NIH 3T3细胞中的荧光定位图像。将管状DNA折纸纳米结构的荧光定位图像与溶酶体的全内反射荧光（TIRF）图像相结合，揭示了这两种结构之间的详细相互作用。定量分析表明，随着时间的推移，管状折纸可以被溶酶体捕获并降解。
Zotero 插件