Martins João, Ares Aitana, Casais Vinicius, Costa Joana, Canhoto Jorge
Department of Life Sciences, Centre for Functional Ecology, University of Coimbra, Calçada Martim de Freitas, 3000-456 Coimbra, Portugal.
Laboratory for Phytopathology, Instituto Pedro Nunes, 3030-199 Coimbra, Portugal.
Plants (Basel). 2021 Jul 30;10(8):1569. doi: 10.3390/plants10081569.
L. is a resilient tree with a circum-Mediterranean distribution. Besides its ecological relevance, it is vital for local economies as a fruit tree. Several microorganisms are responsible for strawberry tree diseases, leading to production constrictions. Thus, the development of alternative plant protection strategies is necessary, such as bacterial endophytes, which may increase their host's overall fitness and productivity. As agricultural practices are a driving factor of microbiota, this paper aimed to isolate, identify and characterize endophytic bacteria from strawberry tree leaves from plants growing spontaneously in a natural environment as well as from plants growing in orchards. A total of 62 endophytes were isolated from leaves and identified as , , , and . Although a slightly higher number of species was found in wild plants, no differences in terms of diversity indexes were found. Sixteen isolates were tested in vitro for their antagonistic effect against mycopathogens. was the most effective antagonist causing a growth reduction of 20% in and 40% in and . Several endophytic isolates also exhibited plant growth-promoting potential. This study provides insights into the diversity of endophytic bacteria in leaves and their potential role as growth promoters and pathogen antagonists.
草莓树是一种适应性强的树木，分布于地中海周边地区。除了具有生态意义外，作为一种果树，它对当地经济至关重要。几种微生物会导致草莓树患病，从而造成产量受限。因此，有必要开发替代植物保护策略，比如细菌内生菌，它们可能会提高宿主的整体健康状况和生产力。由于农业实践是微生物群的一个驱动因素，本文旨在从自然环境中自然生长的植物以及果园中种植的植物的草莓树叶片中分离、鉴定和表征内生细菌。总共从叶片中分离出62种内生菌，并鉴定为[具体菌种]、[具体菌种]、[具体菌种]、[具体菌种]和[具体菌种]。虽然在野生植物中发现的菌种数量略多，但在多样性指数方面未发现差异。对16株分离菌进行了体外测试，以检测它们对[具体真菌病原体]的拮抗作用。[具体菌株]是最有效的拮抗剂，导致[具体真菌病原体1]的生长减少20%，[具体真菌病原体2]的生长减少40%。几种内生分离菌还表现出促进植物生长的潜力。这项研究深入了解了草莓树叶片内生细菌的多样性及其作为生长促进剂和病原体拮抗剂的潜在作用。
