微小RNA-199b-5p通过抑制糖原合成酶激酶-3β/β-连环蛋白信号通路调节骨髓间充质干细胞成骨作用。
miR-199b-5p modulates BMSC osteogenesis via suppressing GSK-3β/β-catenin signaling pathway.
作者信息
Zhao Ruibo, Li Yusheng, Lin Zhangyuan, Wan Jun, Xu Can, Zeng Yong, Zhu Yong
机构信息
Department of Orthopedics, Xiangya Hospital Central South University, PR China.
Department of Orthopedics, Xiangya Hospital Central South University, PR China.
出版信息
Biochem Biophys Res Commun. 2016 Sep 2;477(4):749-754. doi: 10.1016/j.bbrc.2016.06.130. Epub 2016 Jun 27.
miR-199b-5p is up-regulated significantly during the osteogenesis process in human bone marrow stromal cells (BMSCs). Inhibiting miR-199b-5p notably reduces while over-expressing miR-199b-5p promotes the BMSCs osteoblast differentiation, suggested by the alternations of osteogenic genes expression, ALP activity and the ARS-stained mineral nodules. miR-199b-5p exerts its role in BMSC osteogenesis most probably through the GSK-3β/β-catenin signaling pathway. In conclusion, the present study revealed for the first time that miR-199b-5p plays a positive role in osteoblast differentiation.
在人骨髓间充质干细胞（BMSCs）的成骨过程中，miR-199b-5p显著上调。通过成骨基因表达、碱性磷酸酶（ALP）活性以及茜素红染色的矿化结节的变化表明，抑制miR-199b-5p可显著降低BMSCs向成骨细胞的分化，而过表达miR-199b-5p则促进这种分化。miR-199b-5p很可能通过GSK-3β/β-连环蛋白信号通路在BMSCs成骨过程中发挥作用。总之，本研究首次揭示miR-199b-5p在成骨细胞分化中起积极作用。
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