Institut Curie, PSL Research University, CNRS UMR3347, INSERM U1021, 91405, Orsay, France.
Université Paris-Sud, Université Paris-Saclay, rue Georges Clémenceau, 91405 Orsay, France.
Sci Rep. 2016 Jul 1;6:29098. doi: 10.1038/srep29098.
Cold atmospheric pressure plasmas (CAPPs) have emerged over the last decade as a new promising therapy to fight cancer. CAPPs' antitumor activity is primarily due to the delivery of reactive oxygen and nitrogen species (RONS), but the precise determination of the constituents linked to this anticancer process remains to be done. In the present study, using a micro-plasma jet produced in helium (He), we demonstrate that the concentration of H2O2, NO2(-) and NO3(-) can fully account for the majority of RONS produced in plasma-activated buffer. The role of these species on the viability of normal and tumour cell lines was investigated. Although the degree of sensitivity to H2O2 is cell-type dependent, we show that H2O2 alone cannot account for the toxicity of He plasma. Indeed, NO2(-), but not NO3(-), acts in synergy with H2O2 to enhance cell death in normal and tumour cell lines to a level similar to that observed after plasma treatment. Our findings suggest that the efficiency of plasma treatment strongly depends on the combination of H2O2 and NO2(-) in determined concentrations. We also show that the interaction of the He plasma jet with the ambient air is required to generate NO2(-) and NO3(-) in solution.
在过去的十年中，冷等离体子体（CAPPs）作为一种新的有前途的癌症治疗方法而出现。CAPPs 的抗肿瘤活性主要归因于活性氧和氮物种（RONS）的传递，但与这种抗癌过程相关的成分的精确确定仍有待完成。在本研究中，我们使用氦（He）产生的微等离子体射流证明了 H2O2、NO2(-) 和 NO3(-) 的浓度可以完全说明等离子体激活缓冲液中产生的大多数 RONS。研究了这些物质对正常和肿瘤细胞系活力的作用。尽管对 H2O2 的敏感性程度取决于细胞类型，但我们表明 H2O2 本身不能说明 He 等离子体的毒性。事实上，NO2(-) 而不是 NO3(-) 与 H2O2 协同作用，可增强正常和肿瘤细胞系的细胞死亡程度，达到与等离子体处理后观察到的水平相似。我们的研究结果表明，等离子体处理的效率强烈取决于确定浓度下 H2O2 和 NO2(-) 的组合。我们还表明，需要 He 等离子体射流与环境空气相互作用才能在溶液中生成 NO2(-) 和 NO3(-)。
