Sharma Anil Kumar, Venkatesan Gnanavel, Mathesh Karikalan, Ram Hira, Ramakrishnan Muthanan Andavar, Pandey Awadh Bihari
Centre for Wildlife, Conservation, Management and Disease Surveillance, ICAR-Indian Veterinary Research Institute, Izatnagar, Distt. Bareilly, Uttar Pradesh 243122 India.
Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar. Distt. Nainital, Uttarakhand 263 138 India.
Virusdisease. 2016 Jun;27(2):198-202. doi: 10.1007/s13337-016-0316-x. Epub 2016 Apr 28.
A carcass of male free ranging adult blackbuck (Antilope cervicapra) was presented for necropsy examination exhibiting thick confluent nodular skin lesions around the mouth and the dry scaly crusts/fissures on the skin of abdomen, thigh and shoulder with subcutaneous haemorrhages. The skin sample around mouth was found positive for orf virus (ORFV) identified by counterimmunoelectrophoresis and PCR. Histopathology of the mouth skin revealed the hyperkeratinization, epidermal sloughing and epithelial hyperplasia showing acanthosis with rete ridges and few eosinophilic intracytoplasmic inclusion bodies in keratinocytes. Further, comparative B2L gene sequence analysis revealed that the virus isolate from blackbuck had shown 97.8-99.6 and 97.6-99.5 % identity at nucleotide and amino acid levels respectively with Indian isolates and maximum identity with ORFV 79/04, an isolate from India. Phylogenetic analysis based on B2L gene also revealed the same evolutionary relationship that it is closely related to Indian isolates. This seems to be the first report of orf in blackbuck from Indian subcontinent.
一只成年雄性野生黑羚（印度羚）的尸体被送来进行尸检，其口周有密集的厚结节性皮肤病变，腹部、大腿和肩部皮肤有干燥的鳞片状结痂/裂隙，并伴有皮下出血。通过对流免疫电泳和聚合酶链反应鉴定，发现口周皮肤样本中的羊口疮病毒（ORFV）呈阳性。口周皮肤的组织病理学检查显示有角化过度、表皮脱落和上皮增生，表现为棘层增厚和 rete 嵴，角质形成细胞中有少量嗜酸性胞质内包涵体。此外，B2L 基因序列比较分析表明，从黑羚分离出的病毒在核苷酸和氨基酸水平上与印度分离株的同源性分别为 97.8 - 99.6%和 97.6 - 99.5%，与来自印度的分离株 ORFV 79/04 的同源性最高。基于 B2L 基因的系统发育分析也显示出相同的进化关系，即它与印度分离株密切相关。这似乎是印度次大陆黑羚感染羊口疮的首次报道。
