Department of Virology, Veterinary Faculty, Hatay Mustafa Kemal University, Antakya, 31040 Hatay, Turkey.
Comp Immunol Microbiol Infect Dis. 2019 Aug;65:1-6. doi: 10.1016/j.cimid.2019.03.013. Epub 2019 Mar 26.
Orf virus (ORFV) causes contagious skin disease that mainly affects sheep and goats with zoonotic potential. However, there is not enough information about the association between ORFV and occurrence of skin disease in cattle. The present study describes outbreaks of ORFV infection in cattle in different provinces that are located in the Aegean, Central Anatolian and Mediterranean regions of Turkey. During the months of June and August 2017, vesicular fluid and scab samples were collected from cattle which had proliferative skin lesions. First, presence of lumpy skin disease virus (LSDV) and bovine herpesvirus 2 (BoHV-2, known as the causative agent of pseudo-lumpy skin disease) were investigated by real time PCR and PCR, respectively. Then, samples tested for the presence of parapoxviruses by PCR using primers specific to major envelope protein gene (B2L). Parapoxvirus DNA was detected in investigated samples whereas LSDV and BoHV-2 DNA were not detected. The analysis of the B2L gene sequences revealed that cattle were infected with ORFV. The isolates in the present study shared 100% sequence identity at the nucleotide and amino acid level when compared with previously characterised Turkish field ORFV isolates from goats in 2016. Results of the study show unusual infection of cattle with ORFV, and suggest that ORFV jumps the host species barrier from goats to cattle.
口疮病毒（ORFV）可引起具有动物传染倾向的传染性皮肤疾病，主要影响绵羊和山羊。然而，目前对于 ORFV 与牛皮肤疾病发生之间的关联，还没有足够的信息。本研究描述了 2017 年 6 月至 8 月期间在土耳其爱琴海、中安纳托利亚和地中海地区不同省份暴发的牛 ORFV 感染。对具有增殖性皮肤病变的牛采集水疱液和痂皮样本。首先，通过实时 PCR 和 PCR 分别检测了水疱性口炎病毒（LSDV）和牛疱疹病毒 2（BoHV-2，已知是伪牛结节疹的病原体）的存在。然后，使用针对主要包膜蛋白基因（B2L）的引物通过 PCR 检测副痘病毒。在所研究的样本中检测到副痘病毒 DNA，而 LSDV 和 BoHV-2 DNA 未被检测到。B2L 基因序列分析表明牛感染了 ORFV。与 2016 年从山羊中分离到的先前表征的土耳其田间 ORFV 分离株相比，本研究中的分离株在核苷酸和氨基酸水平上均具有 100％的序列同一性。研究结果表明牛发生了不寻常的 ORFV 感染，并提示 ORFV 从山羊跨越宿主种间屏障感染了牛。
