Singh Manmohan, Raghunathan Raksha, Piazza Victor, Davis-Loiacono Anjul M, Cable Alex, Vedakkan Tegy J, Janecek Trevor, Frazier Michael V, Nair Achuth, Wu Chen, Larina Irina V, Dickinson Mary E, Larin Kirill V
Department of Biomedical Engineering, University of Houston, 3605 Cullen Boulevard, Houston, 77204, USA.
Molecular Physiology and Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, 77584, USA.
Biomed Opt Express. 2016 May 19;7(6):2295-310. doi: 10.1364/BOE.7.002295. eCollection 2016 Jun 1.
We present an analysis of imaging murine embryos at various embryonic developmental stages (embryonic day 9.5, 11.5, and 13.5) by optical coherence tomography (OCT) and optical projection tomography (OPT). We demonstrate that while OCT was capable of rapid high-resolution live 3D imaging, its limited penetration depth prevented visualization of deeper structures, particularly in later stage embryos. In contrast, OPT was able to image the whole embryos, but could not be used in vivo because the embryos must be fixed and cleared. Moreover, the fixation process significantly altered the embryo morphology, which was quantified by the volume of the eye-globes before and after fixation. All of these factors should be weighed when determining which imaging modality one should use to achieve particular goals of a study.
我们展示了通过光学相干断层扫描（OCT）和光学投影断层扫描（OPT）对处于不同胚胎发育阶段（胚胎第9.5天、11.5天和13.5天）的小鼠胚胎进行成像分析。我们证明，虽然OCT能够进行快速高分辨率的实时三维成像，但其有限的穿透深度阻碍了对更深层结构的可视化，特别是在后期胚胎中。相比之下，OPT能够对整个胚胎进行成像，但由于胚胎必须固定和透明化处理，所以不能用于活体成像。此外，固定过程显著改变了胚胎形态，通过固定前后眼球的体积进行了量化。在确定应使用哪种成像方式来实现特定研究目标时，所有这些因素都应予以权衡。
