Zhang Meng, Yu Qilin, Liang Chen, Liu Zhe, Zhang Biao, Li Mingchun
Ministry of Education, Key Laboratory of Molecular Microbiology and Technology, Department of Microbiology, College of Life Science, Nankai University, Tianjin 300071, PR China.
Tianjin Traditional Chinese Medicine University, Tianjin 300193, PR China.
Ecotoxicol Environ Saf. 2016 Oct;132:372-8. doi: 10.1016/j.ecoenv.2016.06.031. Epub 2016 Jul 1.
During the past couple of years, graphene nanomaterials were extremely popular among the scientists due to the promising properties in many aspects. Before the materials being well applied, we should first focus on their biosafety and toxicity. In this study, we investigated the toxicity of synthesized graphene oxide (GO) against the model industrial organism Pichia pastoris. We found that the synthesized GO showed dose-dependent toxicity to P. pastoris, through cell membrane damage and intracellular reactive oxygen species (ROS) accumulation. In response to these cell stresses, cells had normal unsaturated fatty acid (UFA) levels but increased contents of polyunsaturated fatty acid (PUFA) with up-regulation of UFA synthesis-related genes on the transcriptional level, which made it overcome the stress under GO attack. Two UFA defective strains (spt23Δ and fad12Δ) were used to demonstrate the results above. Hence, this study suggested a close connection between PUFAs and cell survival against GO.
在过去几年中，石墨烯纳米材料因其在许多方面具有良好的性能而在科学家中极为流行。在这些材料得到良好应用之前，我们首先应该关注它们的生物安全性和毒性。在本研究中，我们研究了合成氧化石墨烯（GO）对模式工业生物巴斯德毕赤酵母的毒性。我们发现，合成的GO对毕赤酵母表现出剂量依赖性毒性，通过细胞膜损伤和细胞内活性氧（ROS）积累。针对这些细胞应激，细胞具有正常的不饱和脂肪酸（UFA）水平，但多不饱和脂肪酸（PUFA）含量增加，且UFA合成相关基因在转录水平上调，这使其能够在GO攻击下克服应激。使用两个UFA缺陷菌株（spt23Δ和fad12Δ）来证明上述结果。因此，本研究表明PUFA与细胞在GO攻击下的存活密切相关。
