Department of Pediatrics, Taoyuan General Hospital, Taoyuan, Taiwan.
Hsin Sheng Junior College of Medical Care and Management, Taoyuan, Taiwan.
Inflamm Res. 2016 Oct;65(10):795-801. doi: 10.1007/s00011-016-0961-y. Epub 2016 Jul 6.
Asthma is a chronic airway inflammatory disease that has a high prevalence nowadays, and seeking the means of relieving asthmatic symptoms is now an issue with increased importance. While mesenchymal stem cells have been demonstrated to display immunomodulatory effects, the effect of fetus-type mesenchymal stem cells (MSCs) on asthmatic symptoms in vivo have not been reported to date.
Female BALB/c mice at 8 weeks of age were sensitized by ovalbumin, and MSCs derived from Wharton's jelly of human umbilical cord mesenchymal stem cells (hUCMSCs) were injected into the asthmatic mice. Airway hyper-responsiveness, lung eosinophil infiltration, cytokine level in splenocyte cultures and serum immunoglobulin level were measured. Enzyme-linked immunosorbent assay was used to determine cytokine and immunoglobulin levels.
This current study demonstrated that hUCMSCs attenuated both lung lymphocyte and eosinophil infiltration, and significantly decreased the concentration of Th2 cytokines interleukin-5 in splenocyte cultures.
Human umbilical cord mesenchymal stem cells have the advantage of being easily harvested non-invasively and are capable of rapid proliferation, therefore an ideal material for stem cell-based immune therapies. The current study showed that fetal-type MSCs were able to suppress asthmatic symptoms efficiently, and its immunomodulatory effect resulted primarily from suppressing the Th2 pathway in the animal model. This study suggested that hUCMSCs could be an ideal candidate for cell-based therapies of asthma.
哮喘是一种慢性气道炎症性疾病，如今发病率很高，寻求缓解哮喘症状的方法变得越来越重要。间充质干细胞具有免疫调节作用，但胎儿型间充质干细胞（MSCs）对体内哮喘症状的影响尚未见报道。
8 周龄雌性 BALB/c 小鼠用卵清蛋白致敏，将人脐带间充质干细胞（hUCMSCs）衍生的羊水型 MSCs 注射到哮喘小鼠体内。测量气道高反应性、肺嗜酸性粒细胞浸润、脾细胞培养中细胞因子水平和血清免疫球蛋白水平。酶联免疫吸附试验用于测定细胞因子和免疫球蛋白水平。
本研究表明，hUCMSCs 减轻了肺淋巴细胞和嗜酸性粒细胞浸润，并显著降低了脾细胞培养中 Th2 细胞因子白细胞介素-5 的浓度。
人脐带间充质干细胞具有易于非侵入性采集和快速增殖的优势，因此是基于干细胞的免疫治疗的理想材料。本研究表明，胎儿型 MSCs 能够有效抑制哮喘症状，其免疫调节作用主要来自于抑制动物模型中的 Th2 途径。本研究提示 hUCMSCs 可能是哮喘细胞治疗的理想候选物。
