Department of Polymer Engineering and Technology, University of the Punjab, Lahore, Pakistan.
Department of Metallurgy and Materials Engineering, PIEAS, Nilore, Islamabad, Pakistan.
Int J Biol Macromol. 2016 Nov;92:1-10. doi: 10.1016/j.ijbiomac.2016.07.010. Epub 2016 Jul 4.
Novel silane crosslinked tough hydrogel scaffolds were prepared using chitosan (CS) and polyvinyl alcohol (PVA) to give network structure and scaffolds properties. The influence of crosslinking and PVA concentration on scaffolds were studied. Fourier transform infrared spectroscopy (FTIR) spectroscopy confirmed the presence of incorporated components. Tensile strength (TS) and fracture strain analysis of scaffolds were detected owing to the mutual effect of chemically and physically crosslinked network. Tough hydrogel scaffolds having 90% CS and 10% PVA exhibited TS of 49.18MPa and 10.15% elongation at break. The contact angle is less than 90 exhibited the hydrophilic nature of the scaffold. X-ray diffraction analysis (XRD) indicated the characteristics peaks fitting to CS and PVA and increase in the crystallinity of scaffolds. Cytotoxicity of scaffolds with different human fibroblast cell lines (F121, F192 and F84) for indirect method and human dermal fibroblast cell lines (F121) for direct method was evaluated. This indicated that these biomaterials were non-toxic, viable to the used cell lines, helpful in the growth of these cells and did not discharge toxic material damaging to the living cells.
新型硅烷交联坚韧水凝胶支架采用壳聚糖（CS）和聚乙烯醇（PVA）制备，赋予其网络结构和支架性能。研究了交联和 PVA 浓度对支架的影响。傅里叶变换红外光谱（FTIR）证实了掺入成分的存在。由于化学和物理交联网络的相互作用，检测了支架的拉伸强度（TS）和断裂伸长率分析。具有 90%CS 和 10%PVA 的坚韧水凝胶支架的 TS 为 49.18MPa，断裂伸长率为 10.15%。接触角小于 90 度表明支架具有亲水性。X 射线衍射分析（XRD）表明特征峰与 CS 和 PVA 拟合，并增加了支架的结晶度。用间接法测定了不同人成纤维细胞系（F121、F192 和 F84）和直接法测定了人真皮成纤维细胞系（F121）的支架细胞毒性。这表明这些生物材料无毒，对所用细胞系具有活力，有助于这些细胞的生长，并且不会释放出对活细胞有害的有毒物质。
