Gallagher R B, Cervi P, Kelly J, Dolan C, Weir D G, Feighery C
Department of Immunology, Trinity College Medical School, Dublin, Ireland.
J Clin Lab Immunol. 1989 Mar;28(3):115-21.
In a randomly selected group of coeliac patients, alpha-gliadin antibodies (AGA) of subclasses IgG1 and IgG3 were significantly elevated (p less than 0.01) in comparison to both normal and disease control groups. This result was, however, influenced by increased total AGA levels in the coeliac patients. AGA subclass profiles of cohorts of coeliac and normal controls matched for total IgG AGA were therefore compared. It was found that IgG3 AGA levels were higher in the coeliac group (p = 0.006) while IgG4 titres were higher in the control group (p = 0.005). Titres of IgG1 and IgG2 AGA did not differ between the two groups. Because of the marked differences between the ability of IgG3 and IgG4 to activate complement, alpha-gliadin-specific complement fixation was measured. Using randomly selected coeliac and normal sera, the patient group activated highly significantly greater quantities of complement than controls (p less than 0.01). Furthermore, when samples matched for total IgG AGA were compared, sera from coeliac patients were again found to activate greater amounts of complement than sera from controls (p = 0.024). Thus AGA in coeliac sera are both structurally and functionally distinguishable from AGA in normal control sera.
在一组随机挑选的乳糜泻患者中，与正常对照组和疾病对照组相比，IgG1和IgG3亚类的α-麦醇溶蛋白抗体（AGA）显著升高（p<0.01）。然而，这一结果受到乳糜泻患者总AGA水平升高的影响。因此，对总IgG AGA匹配的乳糜泻患者队列和正常对照队列的AGA亚类谱进行了比较。结果发现，乳糜泻组的IgG3 AGA水平较高（p = 0.006），而对照组的IgG4滴度较高（p = 0.005）。两组之间的IgG1和IgG2 AGA滴度没有差异。由于IgG3和IgG4激活补体的能力存在显著差异，因此对α-麦醇溶蛋白特异性补体固定进行了检测。使用随机挑选的乳糜泻患者和正常血清，患者组激活的补体数量比对照组高得多（p<0.01）。此外，当比较总IgG AGA匹配的样本时，再次发现乳糜泻患者的血清比对照组的血清激活更多的补体（p = 0.024）。因此，乳糜泻血清中的AGA在结构和功能上与正常对照血清中的AGA有所不同。
